Extracellular matrix proteins produced by stromal cells in idiopathic pulmonary fibrosis and lung adenocarcinoma.
Adenocarcinoma of Lung
/ genetics
Aged
Cell Adhesion Molecules
/ genetics
Cells, Cultured
Collagen Type IV
/ genetics
Extracellular Matrix
/ metabolism
Extracellular Matrix Proteins
/ metabolism
Female
Fibroblasts
/ metabolism
Gene Expression
/ genetics
Gene Expression Profiling
/ methods
Gene Expression Regulation
/ genetics
Humans
Idiopathic Pulmonary Fibrosis
/ genetics
Lung
/ pathology
Lung Neoplasms
/ metabolism
Male
Matrix Metalloproteinase 1
/ genetics
Matrix Metalloproteinase 3
/ genetics
Middle Aged
Signal Transduction
Stromal Cells
/ metabolism
Transcriptome
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
19
11
2020
accepted:
30
03
2021
entrez:
27
4
2021
pubmed:
28
4
2021
medline:
13
10
2021
Statut:
epublish
Résumé
Idiopathic pulmonary fibrosis (IPF) and lung cancer share common risk factors, epigenetic and genetic alterations, the activation of similar signaling pathways and poor survival. The aim of this study was to examine the gene expression profiles of stromal cells from patients with IPF and lung adenocarcinoma (ADC) as well as from normal lung. The gene expression levels of cultured stromal cells derived from non-smoking patients with ADC from the tumor (n = 4) and the corresponding normal lung (n = 4) as well as from patients with IPF (n = 4) were investigated with Affymetrix microarrays. The expression of collagen type IV alpha 1 chain, periostin as well as matrix metalloproteinase-1 and -3 in stromal cells and lung tissues were examined with quantitative real-time reverse transcriptase polymerase chain reaction and immunohistochemistry, respectively. Twenty genes were similarly up- or down-regulated in IPF and ADC compared to control, while most of the altered genes in IPF and ADC were differently expressed, including several extracellular matrix genes. Collagen type IV alpha 1 chain as well as matrix metalloproteinases-1 and -3 were differentially expressed in IPF compared to ADC. Periostin was up-regulated in both IPF and ADC in comparison to control. All studied factors were localized by immunohistochemistry in stromal cells within fibroblast foci in IPF and stroma of ADC. Despite the similarities found in gene expressions of IPF and ADC, several differences were also detected, suggesting that the molecular changes occurring in these two lung illnesses are somewhat different.
Identifiants
pubmed: 33905434
doi: 10.1371/journal.pone.0250109
pii: PONE-D-20-36430
pmc: PMC8078755
doi:
Substances chimiques
Cell Adhesion Molecules
0
Collagen Type IV
0
Extracellular Matrix Proteins
0
POSTN protein, human
0
Matrix Metalloproteinase 3
EC 3.4.24.17
MMP1 protein, human
EC 3.4.24.7
Matrix Metalloproteinase 1
EC 3.4.24.7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0250109Déclaration de conflit d'intérêts
I have read the journal’s policy and the authors of this manuscript have the following competing interests: R.K. has received a congress travel stipend from Orion Pharma, a consulting fee from Boehringer-Ingelheim, and lecture fees from Roche and Boehringer-Ingelheim. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
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