The anti-Rac1-GTP antibody and the detection of active Rac1: a tool with a fundamental flaw.
Anti-Rac1-GTP antibody
Rac1
Rho GTPases
cancer cells
immunofluorescence
vimentin
Journal
Small GTPases
ISSN: 2154-1256
Titre abrégé: Small GTPases
Pays: United States
ID NLM: 101530974
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
pubmed:
30
4
2021
medline:
1
12
2022
entrez:
29
4
2021
Statut:
ppublish
Résumé
Rac1 is a member of the Rho GTPase family and is involved in many cellular processes, particularly the formation of actin-rich membrane protrusions, such as lamellipodia and ruffles. With such a widely studied protein, it is essential that the research community has reliable tools for detecting Rac1 activation both in cellular models and tissues. Using a series of cancer cellular models, we recently demonstrated that a widely used antibody for visualizing active Rac1 (Rac1-GTP) does not recognize Rac1 but instead recognizes vimentin filaments (Baker MJ, J. Biol. Chem. 295:13698-13710, 2020). We believe that this tool has misled the field and impose on the GTPase research community the need to validate published results using this antibody as well as to continue the development of new resources to visualize endogenous active Rac1.
Identifiants
pubmed: 33910489
doi: 10.1080/21541248.2021.1920824
pmc: PMC9707529
doi:
Substances chimiques
cdc42 GTP-Binding Protein
EC 3.6.5.2
rac1 GTP-Binding Protein
EC 3.6.5.2
rho GTP-Binding Proteins
EC 3.6.5.2
Actins
0
Guanosine Triphosphate
86-01-1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
136-140Subventions
Organisme : NCI NIH HHS
ID : R01 CA189765
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA196232
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01 ES026023
Pays : United States
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