Primary mouse osteoblast and osteoclast culturing and analysis.

Animals Mice Osteoblasts / cytology Osteoclasts / cytology Primary Cell Culture
Cell Biology Cell Differentiation Cell culture Cell isolation Metabolism

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
18 06 2021
Historique:
entrez: 29 4 2021
pubmed: 30 4 2021
medline: 30 4 2021
Statut: epublish

Résumé

Mesenchymal-derived osteoblasts play a key role in bone formation via synthesis and mineralization of the bone and bone remodeling. Osteoclasts are multinucleated cells of hematopoietic origin with a role in bone resorption. Here, we describe a protocol for generating primary cultures of these two cell types from bone tissue including the femur, tibia, and humerus of young mice. We describe methods for addressing their activity and/or differentiation, enabling studying the effects of various treatments during or following differentiation

Identifiants

DOI: 10.1016/j.xpro.2021.100452 PMID: 33912848 PMC: PMC8065294
pubmed: 33912848
doi: 10.1016/j.xpro.2021.100452
pii: S2666-1667(21)00159-3
pmc: PMC8065294
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

100452

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

Bio Protoc. 2016 Jun 20;6(12):
pubmed: 28516113
PLoS One. 2017 Jun 7;12(6):e0178960
pubmed: 28591207
Cell Metab. 2020 Oct 6;32(4):575-590.e7
pubmed: 32916104
Biochem Pharmacol. 2003 Oct 1;66(7):1189-98
pubmed: 14505798
BMC Complement Altern Med. 2008 Oct 30;8:59
pubmed: 18973662
Proteome Sci. 2015 May 02;13:16
pubmed: 25969670
ALTEX. 2018;35(1):99-118
pubmed: 28800376

Auteurs

Claire Chevalier (C)

Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
Diabetes Center, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.

Melis Çolakoğlu (M)

Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
Diabetes Center, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.

Julia Brun (J)

Division of Bone Diseases, Geneva University Hospitals, 1211 Geneva, Switzerland.

Cyril Thouverey (C)

Division of Bone Diseases, Geneva University Hospitals, 1211 Geneva, Switzerland.

Nicolas Bonnet (N)

Diabetes Center, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
Nestle Research, Innovation EPFL Park, 1015 Lausanne, Switzerland.

Serge Ferrari (S)

Diabetes Center, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
Division of Bone Diseases, Geneva University Hospitals, 1211 Geneva, Switzerland.

Mirko Trajkovski (M)

Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
Diabetes Center, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Primary mouse osteoblast and osteoclast...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Primary mouse osteoblast and osteoclast...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Ostéoblastes › Primary mouse osteoblast and osteoclast...
questionsmedicales.fr › Cellules › Cellules myéloïdes › Ostéoclastes › Primary mouse osteoblast and osteoclast...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Culture de cellules primaires › Primary mouse osteoblast and osteoclast...