Mechanisms shaping the synergism of zeaxanthin and PsbS in photoprotective energy dissipation in the photosynthetic apparatus of plants.
Arabidopsis
/ metabolism
Arabidopsis Proteins
/ metabolism
Chlorophyll
/ metabolism
Energy Metabolism
Light
Light-Harvesting Protein Complexes
/ metabolism
Microscopy, Fluorescence
Photosynthesis
Photosystem II Protein Complex
/ metabolism
Plant Leaves
/ metabolism
Spectrum Analysis, Raman
Thylakoids
/ metabolism
Zeaxanthins
/ metabolism
PsbS
photoprotection.
photosynthesis
xanthophyll cycle
zeaxanthin
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
revised:
21
04
2021
received:
22
01
2021
accepted:
23
04
2021
pubmed:
30
4
2021
medline:
30
11
2021
entrez:
29
4
2021
Statut:
ppublish
Résumé
Safe operation of photosynthesis is vital to plants and is ensured by the activity of processes protecting chloroplasts against photo-damage. The harmless dissipation of excess excitation energy is considered to be the primary photoprotective mechanism and is most effective in the combined presence of PsbS protein and zeaxanthin, a xanthophyll accumulated in strong light as a result of the xanthophyll cycle. Here we address the problem of specific molecular mechanisms underlying the synergistic effect of zeaxanthin and PsbS. The experiments were conducted with Arabidopsis thaliana, using wild-type plants, mutants lacking PsbS (npq4), and mutants affected in the xanthophyll cycle (npq1), with the application of molecular spectroscopy and imaging techniques. The results lead to the conclusion that PsbS interferes with the formation of densely packed aggregates of thylakoid membrane proteins, thus allowing easy exchange and incorporation of xanthophyll cycle pigments into such structures. It was found that xanthophylls trapped within supramolecular structures, most likely in the interfacial protein region, determine their photophysical properties. The structures formed in the presence of violaxanthin are characterized by minimized dissipation of excitation energy. In contrast, the structures formed in the presence of zeaxanthin show enhanced excitation quenching, thus protecting the system against photo-damage.
Substances chimiques
Arabidopsis Proteins
0
Light-Harvesting Protein Complexes
0
NPQ4 protein, Arabidopsis
0
Photosystem II Protein Complex
0
Zeaxanthins
0
Chlorophyll
1406-65-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
418-433Informations de copyright
© 2021 Society for Experimental Biology and John Wiley & Sons Ltd.
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