Conformation-Specific Inhibitory Anti-MMP-7 Monoclonal Antibody Sensitizes Pancreatic Ductal Adenocarcinoma Cells to Chemotherapeutic Cell Kill.
drug synergy
fas ligand
gemcitabine
matrilysin
matrix metalloproteinase-7
monoclonal antibody
oxaliplatin
pancreatic ductal adenocarcinoma
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
02 Apr 2021
02 Apr 2021
Historique:
received:
23
02
2021
revised:
23
03
2021
accepted:
30
03
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
1
5
2021
Statut:
epublish
Résumé
Matrix metalloproteases (MMPs) undergo post-translational modifications including pro-domain shedding. The activated forms of these enzymes are effective drug targets, but generating potent biological inhibitors against them remains challenging. We report the generation of anti-MMP-7 inhibitory monoclonal antibody (GSM-192), using an alternating immunization strategy with an active site mimicry antigen and the activated enzyme. Our protocol yielded highly selective anti-MMP-7 monoclonal antibody, which specifically inhibits MMP-7's enzyme activity with high affinity (IC
Identifiants
pubmed: 33918254
pii: cancers13071679
doi: 10.3390/cancers13071679
pmc: PMC8038143
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : European Union Framework Programme 7 [FP7/2007-2013] "SaveMe"
ID : 263307
Organisme : Israeli Science Foundation
ID : 1800/19
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : The Thompson Family Foundation, Inc. grant
ID : Targeting Pancreas Cancer
Organisme : European Union's Horizon 2020 research and innovation programme
ID : 801126
Organisme : European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme
ID : 695437
Organisme : Big C
ID : 14-09R
Organisme : The USA-Israel Binational Science Foundation
ID : 712506-01
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