Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.

CRISPR-Associated Proteins / genetics CRISPR-Cas Systems / genetics DNA Mutational Analysis Gene Expression Regulation, Archaeal Haloarcula / genetics Operon RNA, Archaeal / physiology RNA, Transfer, Arg / metabolism Toxin-Antitoxin Systems / genetics

Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
30 04 2021
Historique:
received: 16 09 2020
revised: 29 12 2020
accepted: 10 03 2021
entrez: 30 4 2021
pubmed: 1 5 2021
medline: 14 5 2021
Statut: ppublish

Résumé

CRISPR-Cas systems provide RNA-guided adaptive immunity in prokaryotes. We report that the multisubunit CRISPR effector Cascade transcriptionally regulates a toxin-antitoxin RNA pair, CreTA. CreT (Cascade-repressed toxin) is a bacteriostatic RNA that sequesters the rare arginine tRNA

Identifiants

DOI: 10.1126/science.abe5601 PMID: 33926924
pubmed: 33926924
pii: 372/6541/eabe5601
doi: 10.1126/science.abe5601
pii:
doi:

Substances chimiques

CRISPR-Associated Proteins 0
RNA, Archaeal 0
RNA, Transfer, Arg 0

Types de publication

Journal Article Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Auteurs

Ming Li (M)

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. lim_im@im.ac.cn xiangh@im.ac.cn.
CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Chinese Academy of Sciences, Beijing, China.
College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
ORCID: 0000-0002-0634-8396

Luyao Gong (L)

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
ORCID: 0000-0001-9834-3914

Feiyue Cheng (F)

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
ORCID: 0000-0002-8096-4680

Haiying Yu (H)

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Dahe Zhao (D)

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Rui Wang (R)

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
ORCID: 0000-0003-2127-2251

Tian Wang (T)

College of Life Sciences, Sichuan Normal University, Chengdu, China.

Shengjie Zhang (S)

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
ORCID: 0000-0001-6988-2791

Jian Zhou (J)

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Sergey A Shmakov (SA)

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA.
ORCID: 0000-0002-4243-0709

Eugene V Koonin (EV)

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA.
ORCID: 0000-0003-3943-8299

Hua Xiang (H)

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. lim_im@im.ac.cn xiangh@im.ac.cn.
College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China.
ORCID: 0000-0003-0369-1225

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines associées aux CRISPR › Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Analyse de mutations d'ADN › Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes archéens › Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.
questionsmedicales.fr › Archéobactéries › Euryarchaeota › Halobacteriales › Halobacteriaceae › Haloarcula › Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Opéron › Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN des archées › Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN de transfert › ARN de transfert spécifiques des différents acides aminés › ARN de transfert de l'arginine › Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.
questionsmedicales.fr › Phénomènes microbiologiques › Systèmes toxine-antitoxine › Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.