A plasma membrane transporter coordinates phosphate reallocation and grain filling in cereals.
Animals
Cell Membrane
/ metabolism
Chromosome Mapping
Edible Grain
/ metabolism
Gene Expression Regulation, Plant
HEK293 Cells
Humans
Membrane Transport Proteins
/ metabolism
Mutation
/ genetics
Oryza
/ enzymology
Phenotype
Phosphates
/ metabolism
Plant Proteins
/ genetics
Plants, Genetically Modified
RNA, Messenger
/ genetics
Seeds
/ genetics
Starch
/ biosynthesis
Xenopus
Zea mays
/ genetics
Journal
Nature genetics
ISSN: 1546-1718
Titre abrégé: Nat Genet
Pays: United States
ID NLM: 9216904
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
20
04
2020
accepted:
24
03
2021
pubmed:
1
5
2021
medline:
21
7
2021
entrez:
30
4
2021
Statut:
ppublish
Résumé
Phosphate (Pi) is essential to plant growth and crop yield. However, it remains unknown how Pi homeostasis is maintained during cereal grain filling. Here, we identified a rice grain-filling-controlling PHO1-type Pi transporter, OsPHO1;2, through map-based cloning. Pi efflux activity and its localization to the plasma membrane of seed tissues implicated a specific role for OsPHO1;2 in Pi reallocation during grain filling. Indeed, Pi over-accumulated in developing seeds of the Ospho1;2 mutant, which inhibited the activity of ADP-glucose pyrophosphorylase (AGPase), important for starch synthesis, and the grain-filling defect was alleviated by overexpression of AGPase in Ospho1;2-mutant plants. A conserved function was recognized for the maize transporter ZmPHO1;2. Importantly, ectopic overexpression of OsPHO1;2 enhanced grain yield, especially under low-Pi conditions. Collectively, we discovered a mechanism underlying Pi transport, grain filling and P-use efficiency, providing an efficient strategy for improving grain yield with minimal P-fertilizer input in cereals.
Identifiants
pubmed: 33927398
doi: 10.1038/s41588-021-00855-6
pii: 10.1038/s41588-021-00855-6
doi:
Substances chimiques
Membrane Transport Proteins
0
Phosphates
0
Plant Proteins
0
RNA, Messenger
0
Starch
9005-25-8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
906-915Références
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