Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019.
Aseptic meningitis
Children
Enterovirus (EV)
Liaocheng
Phylogenetic analysis
Journal
BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551
Informations de publication
Date de publication:
01 May 2021
01 May 2021
Historique:
received:
21
12
2020
accepted:
23
04
2021
entrez:
2
5
2021
pubmed:
3
5
2021
medline:
12
5
2021
Statut:
epublish
Résumé
Aseptic meningitis is most often caused by enteroviruses (EVs), but EVs associated with aseptic meningitis have not yet been reported in Liaocheng. The aim of this study was to determine the prevalence and genetic characteristics of EVs causing aseptic meningitis in children in Liaocheng. We reviewed the epidemiological and clinical characteristics of 504 paediatric cases of aseptic meningitis in Liaocheng from 2018 to 2019 and analysed the phylogeny of the predominant EV types causing this disease. A total of 107 children were positive for EV in cerebrospinal fluid samples by nested PCR. Most of the positive patients were children 13 years old or younger and had symptoms such as fever, headache and vomiting (P < 0.05). The seasons with the highest prevalence of EV-positive cases were summer and autumn. The 107 EV sequences belonged to 8 serotypes, and echovirus types 18, 6 and 11 were the three dominant serotypes in Liaocheng during the 2-year study period. Phylogenetic analyses demonstrated that the E18 and E6 isolates belonged to subgenotype C2, while the E11 isolates belonged to subgenotype D5. VP1 analysis suggested that only one lineage of these three types was cocirculating in the Liaocheng region. This study demonstrated the diverse EV genotypes contributing to a large outbreak of aseptic meningitis in Liaocheng. Therefore, large-scale surveillance is required to assess the epidemiology of EVs associated with aseptic meningitis and is important for the diagnosis and treatment of aseptic meningitis in Liaocheng.
Sections du résumé
BACKGROUND
BACKGROUND
Aseptic meningitis is most often caused by enteroviruses (EVs), but EVs associated with aseptic meningitis have not yet been reported in Liaocheng. The aim of this study was to determine the prevalence and genetic characteristics of EVs causing aseptic meningitis in children in Liaocheng.
METHODS
METHODS
We reviewed the epidemiological and clinical characteristics of 504 paediatric cases of aseptic meningitis in Liaocheng from 2018 to 2019 and analysed the phylogeny of the predominant EV types causing this disease.
RESULTS
RESULTS
A total of 107 children were positive for EV in cerebrospinal fluid samples by nested PCR. Most of the positive patients were children 13 years old or younger and had symptoms such as fever, headache and vomiting (P < 0.05). The seasons with the highest prevalence of EV-positive cases were summer and autumn. The 107 EV sequences belonged to 8 serotypes, and echovirus types 18, 6 and 11 were the three dominant serotypes in Liaocheng during the 2-year study period. Phylogenetic analyses demonstrated that the E18 and E6 isolates belonged to subgenotype C2, while the E11 isolates belonged to subgenotype D5. VP1 analysis suggested that only one lineage of these three types was cocirculating in the Liaocheng region.
CONCLUSIONS
CONCLUSIONS
This study demonstrated the diverse EV genotypes contributing to a large outbreak of aseptic meningitis in Liaocheng. Therefore, large-scale surveillance is required to assess the epidemiology of EVs associated with aseptic meningitis and is important for the diagnosis and treatment of aseptic meningitis in Liaocheng.
Identifiants
pubmed: 33933008
doi: 10.1186/s12879-021-06112-9
pii: 10.1186/s12879-021-06112-9
pmc: PMC8088645
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
405Subventions
Organisme : Natural Science Foundation of Shandong Province
ID : ZR2017PH054
Organisme : Shandong Traditional Chinese Medicine Development Project
ID : 2017494
Organisme : Shandong Province Medical and Health Development Plan Project
ID : 2018WS427
Organisme : Shandong Traditional Chinese Medicine Science and Technology Development Plan Project
ID : 2019-0885
Références
BMC Infect Dis. 2019 Nov 29;19(1):1010
pubmed: 31783807
J Neurol Sci. 2019 Mar 15;398:176-183
pubmed: 30731305
J Med Microbiol. 2011 Sep;60(Pt 9):1360-1365
pubmed: 21546563
BMC Infect Dis. 2017 Jul 14;17(1):494
pubmed: 28705180
Bioinformatics. 2014 May 1;30(9):1305-7
pubmed: 24413669
J Clin Microbiol. 2008 Jan;46(1):87-96
pubmed: 17977989
Kansenshogaku Zasshi. 1994 Oct;68(10):1251-5
pubmed: 7996023
BMC Infect Dis. 2018 Dec 29;18(1):709
pubmed: 30594238
Pediatrics. 1968 Jan;41(1):77-90
pubmed: 5635489
Pediatr Infect Dis J. 2016 May;35(5):567-9
pubmed: 26862675
Virus Genes. 2012 Jun;44(3):388-94
pubmed: 22311429
Int J Infect Dis. 2019 Feb;79:58-64
pubmed: 30423458
J Med Virol. 2010 Mar;82(3):441-5
pubmed: 20087933
Microbiol Resour Announc. 2019 Oct 24;8(43):
pubmed: 31649074
Virol J. 2010 Apr 06;7:72
pubmed: 20367886
J Clin Microbiol. 1999 Dec;37(12):3928-33
pubmed: 10565909
J Clin Virol. 2016 Aug;81:90-3
pubmed: 27367546
Arch Virol. 2019 Sep;164(9):2417-2429
pubmed: 31187277
J Clin Virol. 2009 Feb;44(2):119-24
pubmed: 19135410
Infect Genet Evol. 2015 Mar;30:244-248
pubmed: 25562123
Virol J. 2013 Aug 23;10:263
pubmed: 23968330
Handb Clin Neurol. 2014;123:379-416
pubmed: 25015496
PLoS One. 2014 Feb 21;9(2):e89766
pubmed: 24587020
Arch Virol. 2020 Feb;165(2):433-438
pubmed: 31828510
Curr Opin Infect Dis. 2015 Oct;28(5):479-87
pubmed: 26203854
Appl Environ Microbiol. 2015 Apr;81(7):2311-9
pubmed: 25616804
Lancet Infect Dis. 2016 Nov;16(11):1279-1287
pubmed: 27527749
Appl Environ Microbiol. 2012 Oct;78(19):6946-53
pubmed: 22843520
J Hyg (Lond). 1985 Oct;95(2):483-92
pubmed: 4067300
J Med Virol. 2013 Mar;85(3):483-9
pubmed: 23212939
Emerg Infect Dis. 2012 Nov;18(11):1865-7
pubmed: 23092737
Virology. 2018 Mar;516:196-201
pubmed: 29407377
Emerg Infect Dis. 2005 Apr;11(4):562-7
pubmed: 15829194
Pathog Glob Health. 2016 Sep;110(6):233-237
pubmed: 27646838
Bing Du Xue Bao. 2015 Jan;31(1):36-41
pubmed: 25997328
Pediatr Infect Dis J. 2019 Jan;38(1):37-41
pubmed: 30531527
Viruses. 2019 Jul 29;11(8):
pubmed: 31362357
Sci Rep. 2019 Jul 22;9(1):10583
pubmed: 31332200
Arch Virol. 2014 Feb;159(2):379-83
pubmed: 23990054
Prim Care. 2013 Sep;40(3):707-26
pubmed: 23958365
Emerg Microbes Infect. 2017 Jun 21;6(6):e54
pubmed: 28634356
Eur J Pediatr. 2015 Nov;174(11):1549-53
pubmed: 26003661
PLoS One. 2017 Feb 22;12(2):e0172357
pubmed: 28225788
Virology. 2019 May;531:79-92
pubmed: 30856485
Mo Med. 2009 Nov-Dec;106(6):420-4
pubmed: 20063513
Biochemistry (Mosc). 2017 Dec;82(13):1615-1631
pubmed: 29523062
Appl Environ Microbiol. 2011 Jun;77(11):3786-92
pubmed: 21478313
Med Microbiol Immunol. 2016 Oct;205(5):471-83
pubmed: 27369854
Eur J Pediatr. 2008 May;167(5):587-9
pubmed: 17593390
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Dec;21(4):313-5
pubmed: 18322587
Springerplus. 2016 Sep 08;5(1):1515
pubmed: 27652088
Clin Microbiol Infect. 2014 Dec;20(12):1289-96
pubmed: 25039903
Viruses. 2016 Jan 06;8(1):
pubmed: 26751470
Rev Neurol (Paris). 2019 Sep - Oct;175(7-8):475-480
pubmed: 31375286
Epidemiol Infect. 2001 Jun;126(3):441-4
pubmed: 11467801
Bing Du Xue Bao. 2011 Sep;27(5):462-8
pubmed: 21998959
Pediatr Emerg Care. 2018 Nov;34(11):791-796
pubmed: 27898578
Virol J. 2011 Nov 11;8:516
pubmed: 22078806
Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Sep;27(9):793-7
pubmed: 17299967
Virol Sin. 2019 Feb;34(1):50-58
pubmed: 30790201