The effects of L-carnitine on renal function and gene expression of caspase-9 and Bcl-2 in monosodium glutamate-induced rats.
Animals
Antioxidants
/ pharmacology
Apoptosis
/ drug effects
Calcium
/ blood
Carnitine
/ pharmacology
Caspase 9
/ drug effects
Catalase
/ blood
Gene Expression
/ drug effects
Glutathione Peroxidase
/ blood
Humans
Kidney
/ drug effects
Male
Malondialdehyde
/ blood
Phosphorus
/ blood
Proto-Oncogene Proteins c-bcl-2
/ drug effects
Random Allocation
Rats, Sprague-Dawley
Sodium Glutamate
/ toxicity
Superoxide Dismutase
/ blood
Anti‐oxidant
Bcl-2
Caspase-9
Kidney
L-carnitine
MSG
Journal
BMC nephrology
ISSN: 1471-2369
Titre abrégé: BMC Nephrol
Pays: England
ID NLM: 100967793
Informations de publication
Date de publication:
02 05 2021
02 05 2021
Historique:
received:
23
11
2020
accepted:
19
04
2021
entrez:
2
5
2021
pubmed:
3
5
2021
medline:
12
2
2022
Statut:
epublish
Résumé
Monosodium glutamate (MSG) is frequently consumed as a flavor enhancer or food additive. Possible damages induced by MSG effects on some organs have been stated in experimental animal models. The aim of the present study was to evaluate the protective effects of L-carnitine (L-ca) on the renal tissue in MSG-Induced Rats. In this regard, 60 male rats were randomly divided into six groups (n = 10/each): 1 (Control); 2 (sham); 3 (L-carnitine 200 mg/kg b.w); 4 (MSG 3 g/kg b.w); 5 (MSG + L-carnitine 100 mg/kg); and 6 (MSG + L-carnitine 200 mg/kg). After 6 months, the rats were sacrificed, the blood sample collected and the kidneys harvested for evaluation of biochemical analytes, genes expression, and histopathological changes. MSG significantly increased the serum level of MDA, BUN, creatinine, uric acid and renal Caspase-9, NGAL and KIM-1 expression, but it decreased the serum activity also renal expression of SOD, catalase, GPX, and Bcl-2 expression compared to the control group. Treatment with L-ca significantly reduced the serum BUN, creatinine, uric acid and MDA level and increased catalase, GPX and SOD compared to the MSG group. However, only administration of L-ca 200 significantly decreased the caspase-9, NGAL and KIM-1; also, it increased the Bcl-2 expression in the kidney compared to the MSG group. Our findings indicated that L-carnitine had a major impact on the cell protection and might be an effective therapy in ameliorating the complications of the kidney induced by MSG via its antioxidant and anti-apoptotic properties.
Sections du résumé
BACKGROUND
Monosodium glutamate (MSG) is frequently consumed as a flavor enhancer or food additive. Possible damages induced by MSG effects on some organs have been stated in experimental animal models. The aim of the present study was to evaluate the protective effects of L-carnitine (L-ca) on the renal tissue in MSG-Induced Rats.
METHODS
In this regard, 60 male rats were randomly divided into six groups (n = 10/each): 1 (Control); 2 (sham); 3 (L-carnitine 200 mg/kg b.w); 4 (MSG 3 g/kg b.w); 5 (MSG + L-carnitine 100 mg/kg); and 6 (MSG + L-carnitine 200 mg/kg). After 6 months, the rats were sacrificed, the blood sample collected and the kidneys harvested for evaluation of biochemical analytes, genes expression, and histopathological changes.
RESULTS
MSG significantly increased the serum level of MDA, BUN, creatinine, uric acid and renal Caspase-9, NGAL and KIM-1 expression, but it decreased the serum activity also renal expression of SOD, catalase, GPX, and Bcl-2 expression compared to the control group. Treatment with L-ca significantly reduced the serum BUN, creatinine, uric acid and MDA level and increased catalase, GPX and SOD compared to the MSG group. However, only administration of L-ca 200 significantly decreased the caspase-9, NGAL and KIM-1; also, it increased the Bcl-2 expression in the kidney compared to the MSG group.
CONCLUSIONS
Our findings indicated that L-carnitine had a major impact on the cell protection and might be an effective therapy in ameliorating the complications of the kidney induced by MSG via its antioxidant and anti-apoptotic properties.
Identifiants
pubmed: 33933022
doi: 10.1186/s12882-021-02364-4
pii: 10.1186/s12882-021-02364-4
pmc: PMC8088661
doi:
Substances chimiques
Antioxidants
0
Bcl2 protein, rat
0
Proto-Oncogene Proteins c-bcl-2
0
Phosphorus
27YLU75U4W
Malondialdehyde
4Y8F71G49Q
Catalase
EC 1.11.1.6
Glutathione Peroxidase
EC 1.11.1.9
Superoxide Dismutase
EC 1.15.1.1
Casp9 protein, rat
EC 3.4.22.-
Caspase 9
EC 3.4.22.-
Carnitine
S7UI8SM58A
Calcium
SY7Q814VUP
Sodium Glutamate
W81N5U6R6U
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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