Development of an in-situ signal amplified electrochemical assay for detection of Listeria monocytogenes with label-free strategy.

Animals Benzidines / chemistry DNA, Catalytic / chemistry Electrochemical Techniques / methods Food Microbiology / methods G-Quadruplexes Listeria monocytogenes / genetics Molecular Diagnostic Techniques / methods Nucleic Acid Amplification Techniques / methods Pork Meat / microbiology Reproducibility of Results Sensitivity and Specificity
G-quadruplex-hemin DNAzyme Listeria monocytogenes Loop-mediated isothermal amplification Screen-printed carbon electrodes

Journal

Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639

Informations de publication

Date de publication:
01 Oct 2021
Historique:
received: 12 08 2020
revised: 13 04 2021
accepted: 16 04 2021
pubmed: 3 5 2021
medline: 8 6 2021
entrez: 2 5 2021
Statut: ppublish

Résumé

Listeria monocytogenes is an important foodborne pathogen, which imposes great burdens on public health. The current methods for detecting L. monocytogene are limited in several ways such as time consuming and lab equipment dependent. In this study, we developed a new electrochemical assay to improve the efficacy. This assay allows us to generate numerous G-quadruplex sequences while loop-mediated isothermal amplification happens. Then, these G-quadruplex sequences form DNAzyme to produce a color change and an electrochemical signal by oxidizing tetramethylbenzidine. This assay could be finished in 2 h, which significantly reduced the detection time. Also, we confirmed the limit of detection of this assay at 6.8 CFU/mL according to 3σ criterion. Our assay shows good sensitivity to detect bacteria range from 52.5 to 5.25 × 10

Identifiants

DOI: 10.1016/j.foodchem.2021.129894 PMID: 33933968
pubmed: 33933968
pii: S0308-8146(21)00900-6
doi: 10.1016/j.foodchem.2021.129894
pii:
doi:

Substances chimiques

Benzidines 0
DNA, Catalytic 0
3,3',5,5'-tetramethylbenzidine 3B3T5CB8EO

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

129894

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Qiming Chen (Q)

School of Life Sciences, Shanghai University, Shanghai 200444, China.

Chenhui Yao (C)

School of Life Sciences, Shanghai University, Shanghai 200444, China.

Cuiyun Yang (C)

Shanghai Customs, Shanghai 200135, China.

Zhanmin Liu (Z)

School of Life Sciences, Shanghai University, Shanghai 200444, China. Electronic address: zhmliu@shu.edu.cn.

Sibao Wan (S)

School of Life Sciences, Shanghai University, Shanghai 200444, China.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Development of an in-situ signal amplified...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures cycliques › Hydrocarbures aromatiques › Dérivés du benzène › Dérivés du biphényle › Benzidines › Development of an in-situ signal amplified...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN catalytique › Development of an in-situ signal amplified...
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Development of an in-situ signal amplified...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Qualité alimentaire › Sécurité des aliments › Contamination des aliments › Microbiologie alimentaire › Development of an in-situ signal amplified...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › G-quadruplexes › Development of an in-situ signal amplified...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes réguliers à Gram positif › Listeria › Listeria monocytogenes › Development of an in-situ signal amplified...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de diagnostic moléculaire › Development of an in-situ signal amplified...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Development of an in-situ signal amplified...
questionsmedicales.fr › Aliments et boissons › Aliments › Viande › Viande rouge › Viande de porc › Development of an in-situ signal amplified...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Development of an in-situ signal amplified...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Development of an in-situ signal amplified...