Hemoglobin fractionation by Sebia Capillarys 2 Flex Piercing System as primary method for evaluation of hemoglobinopathies.
Capillary electrophoresis
Hemoglobin
Hemoglobin variants
High-pressure liquid chromatography
Journal
Clinica chimica acta; international journal of clinical chemistry
ISSN: 1873-3492
Titre abrégé: Clin Chim Acta
Pays: Netherlands
ID NLM: 1302422
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
18
03
2021
revised:
27
04
2021
accepted:
28
04
2021
pubmed:
4
5
2021
medline:
22
6
2021
entrez:
3
5
2021
Statut:
ppublish
Résumé
Hemoglobin fractionation by capillary zone electrophoresis (CE) is becoming a popular method for the identification of hemoglobin variants that can cause hemoglobinopathies. The goal of this study was to compare the performance of capillary electrophoresis using Sebia Capillarys 2 Flex Piercing system (CE-S) with high-pressure liquid chromatography (HPLC) using Primus Ultra2 Resolution Variants System (HPLC-P) as a primary method in hemoglobinopathy work-up. A total of 306 blood specimens submitted for evaluation of hemoglobinopathies were studied using HPLC-P and CE-S. The reference ranges for Hb A, A CE-S provides comparable information to that obtained by HPLC-P and it is a reliable primary method for the evaluation of hemoglobin variants.
Sections du résumé
BACKGROUND
BACKGROUND
Hemoglobin fractionation by capillary zone electrophoresis (CE) is becoming a popular method for the identification of hemoglobin variants that can cause hemoglobinopathies. The goal of this study was to compare the performance of capillary electrophoresis using Sebia Capillarys 2 Flex Piercing system (CE-S) with high-pressure liquid chromatography (HPLC) using Primus Ultra2 Resolution Variants System (HPLC-P) as a primary method in hemoglobinopathy work-up.
METHODS
METHODS
A total of 306 blood specimens submitted for evaluation of hemoglobinopathies were studied using HPLC-P and CE-S.
RESULTS
RESULTS
The reference ranges for Hb A, A
CONCLUSION
CONCLUSIONS
CE-S provides comparable information to that obtained by HPLC-P and it is a reliable primary method for the evaluation of hemoglobin variants.
Identifiants
pubmed: 33939955
pii: S0009-8981(21)00148-0
doi: 10.1016/j.cca.2021.04.023
pii:
doi:
Substances chimiques
Glycated Hemoglobin A
0
Hemoglobins
0
Hemoglobins, Abnormal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
193-197Informations de copyright
Copyright © 2021. Published by Elsevier B.V.