Long Noncoding RNA CTD-2245E15.3 Promotes Anabolic Enzymes ACC1 and PC to Support Non-Small Cell Lung Cancer Growth.
Acetyl-CoA Carboxylase
/ genetics
Animals
Apoptosis
Biomarkers, Tumor
/ genetics
Carcinoma, Non-Small-Cell Lung
/ genetics
Cell Cycle
Cell Movement
Cell Proliferation
Gene Expression Regulation, Neoplastic
Humans
Lung Neoplasms
/ genetics
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Prognosis
Pyruvate Carboxylase
/ genetics
RNA, Long Noncoding
/ genetics
Survival Rate
Tumor Cells, Cultured
Xenograft Model Antitumor Assays
Journal
Cancer research
ISSN: 1538-7445
Titre abrégé: Cancer Res
Pays: United States
ID NLM: 2984705R
Informations de publication
Date de publication:
01 07 2021
01 07 2021
Historique:
received:
05
12
2019
revised:
09
09
2020
accepted:
29
04
2021
pubmed:
5
5
2021
medline:
15
12
2021
entrez:
4
5
2021
Statut:
ppublish
Résumé
Long noncoding RNAs (lncRNA) have been shown to play critical regulatory roles in the onset and progression of human cancers. However, the functions of a large proportion of lncRNAs are still unexplored. Here we describe a novel lncRNA, CTD-2245E15.3, that promotes lung tumorigenesis by regulating the anabolic enzymes acetyl-CoA carboxylase 1 (ACC1, encoded by the ACACA gene) and pyruvate carboxylase (PC). Differentially expressed lncRNAs between non-small cell lung cancer (NSCLC) and paired adjacent nontumor tissues were identified by a microarray and validated using quantitative real-time polymerase chain reaction. CTD-2245E15.3 was significantly upregulated in NSCLC and was mainly located in the cytoplasm. Knockdown of CTD-2245E15.3 by specific antisense oligonucleotides suppressed cell growth
Identifiants
pubmed: 33941610
pii: 0008-5472.CAN-19-3806
doi: 10.1158/0008-5472.CAN-19-3806
doi:
Substances chimiques
Biomarkers, Tumor
0
RNA, Long Noncoding
0
Pyruvate Carboxylase
EC 6.4.1.1
ACACA protein, human
EC 6.4.1.2
Acetyl-CoA Carboxylase
EC 6.4.1.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3509-3524Informations de copyright
©2021 American Association for Cancer Research.
Références
Beermann J, Piccoli M-T, Viereck J, Thum T. Non-coding RNAs in development and disease: background, mechanisms, and therapeutic approaches. Physiol Rev. 2016;96:1297–325.
Yan X, Hu Z, Feng Y, Hu X, Yuan J, Zhao SD, et al. Comprehensive genomic characterization of long non-coding RNAs across human cancers. Cancer Cell. 2015;28:529–40.
Bhan A, Soleimani M, Mandal SS. Long noncoding RNA and cancer: a new paradigm. Cancer Res. 2017;77:3965–81.
Khalil AM, Guttman M, Huarte M, Garber M, Raj A, Morales DR, et al. Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression. Proc Natl Acad Sci USA. 2009;106:11667–72.
Hung C-L, Wang L-Y, Yu Y-L, Chen H-W, Srivastava S, Petrovics G, et al. A long noncoding RNA connects c-Myc to tumor metabolism. Proc Natl Acad Sci USA. 2014;111:18697–702.
Kallen Amanda N, Zhou X-B, Xu J, Qiao C, Ma J, Yan L, et al. The imprinted H19 LncRNA antagonizes let-7 MicroRNAs. Mol Cell. 2013;52:101–12.
Hanahan D, Weinberg Robert A. Hallmarks of cancer: the next generation. Cell. 2011;144:646–74.
Menendez JA, Lupu R. Fatty acid synthase and the lipogenic phenotype in cancer pathogenesis. Nat Rev Cancer. 2007;7:763.
Currie E, Schulze A, Zechner R, Walther Tobias C, Farese Robert V. Cellular Fatty Acid Metabolism and Cancer. Cell Metab. 2013;18:153–61.
Wakil SJ, Abu-Elheiga LA. Fatty acid metabolism: target for metabolic syndrome. J Lipid Res. 2009;50:S138.
Zhang F, Du G. Dysregulated lipid metabolism in cancer. World J Biol Chem. 2012;3:167–74.
Svensson RU, Parker SJ, Eichner LJ, Kolar MJ, Wallace M, Brun SN, et al. Inhibition of acetyl-CoA carboxylase suppresses fatty acid synthesis and tumor growth of non-small-cell lung cancer in preclinical models. Nat Med. 2016;22:1108.
Pavlova Natalya N, Thompson Craig B. The emerging hallmarks of cancer metabolism. Cell Metab. 2016;23:27–47.
Marin-Valencia I, Yang C, Mashimo T, Cho S, Baek H, Yang X-L, et al. Analysis of tumor metabolism reveals mitochondrial glucose oxidation in genetically diverse human glioblastomas in the mouse brain in vivo. Cell Metab. 2012;15:827–37.
Sellers K, Fox MP, Bousamra M II, Slone SP, Higashi RM, Miller DM, et al. Pyruvate carboxylase is critical for non–small-cell lung cancer proliferation. J Clin Invest. 2015;125:687–98.
Yang F, Zhang L, Huo X-s, Yuan J-h, Xu D, Yuan S-x, et al. Long noncoding RNA high expression in hepatocellular carcinoma facilitates tumor growth through enhancer of zeste homolog 2 in humans. Hepatology. 2011;54:1679–89.
Huang DW, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2008;4:44.
Lan Y, Xiao X, He Z, Luo Y, Wu C, Li L, et al. Long noncoding RNA OCC-1 suppresses cell growth through destabilizing HuR protein in colorectal cancer. Nucleic Acids Res. 2018;46:5809–21.
Wang L, Park HJ, Dasari S, Wang S, Kocher J-P, Li W. CPAT: coding-potential assessment tool using an alignment-free logistic regression model. Nucleic Acids Res. 2013;41:e74–e.
Moessinger C, Kuerschner L, Spandl J, Shevchenko A, Thiele C. Human lysophosphatidylcholine acyltransferases 1 and 2 are located in lipid droplets where they catalyze the formation of phosphatidylcholine. J Biol Chem. 2011;286:21330–9.
Tay Y, Rinn J, Pandolfi PP. The multilayered complexity of ceRNA crosstalk and competition. Nature. 2014;505:344.
Bosson Andrew D, Zamudio Jesse R, Sharp Phillip A. Endogenous miRNA and target concentrations determine susceptibility to potential ceRNA competition. Mol Cell. 2014;56:347–59.
Steinberg GR, Kemp BE. AMPK in health and disease. Physiol Rev. 2009;89:1025–78.
Svensson RU, Shaw RJ. Lipid synthesis is a metabolic liability of non–small cell lung cancer. Cold Spring Harb Symp Quant Biol. 2016;81:93–103.
Blank HM, Perez R, He C, Maitra N, Metz R, Hill J, et al. Translational control of lipogenic enzymes in the cell cycle of synchronous, growing yeast cells. EMBO J. 2017;36:487–502.
Jitrapakdee S, St Maurice M, Rayment I, Cleland WW, Wallace John C, Attwood Paul V. Structure, mechanism and regulation of pyruvate carboxylase. Biochem J. 2008;413:369–87.
Ricciuti B, Mencaroni C, Paglialunga L, Paciullo F, Crinò L, Chiari R, et al. Long noncoding RNAs: new insights into non-small cell lung cancer biology, diagnosis and therapy. Med Oncol. 2016;33:18.
Zhou G, Chen X. Emerging role of extracellular microRNAs and lncRNAs. ExRNA. 2019;1:10.
Chen R, Li W, Sun Y, Duan Y, Li Q, Zhang A, et al. Comprehensive analysis of lncRNA and mRNA expression profiles in lung cancer. Clin Lab. 2017;63:313–20.
Li K, Sun D, Gou Q, Ke X, Gong Y, Zuo Y, et al. Long non-coding RNA linc00460 promotes epithelial-mesenchymal transition and cell migration in lung cancer cells. Cancer Lett. 2018;420:80–90.
Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature. 2010;464:1071.
Xu T-p, Huang M-d, Xia R, Liu X-x, Sun M, Yin L, et al. Decreased expression of the long non-coding RNA FENDRR is associated with poor prognosis in gastric cancer and FENDRR regulates gastric cancer cell metastasis by affecting fibronectin1 expression. J Hematol Oncol. 2014;7:63.
Chen L-L. Linking long noncoding RNA localization and function. Trends Biochem Sci. 2016;41:761–72.
Vander Heiden MG, Cantley LC, Thompson CB. Understanding the warburg effect: the metabolic requirements of cell proliferation. Science. 2009;324:1029–33.
Li J, Cheng J-X. Direct visualization oflipogenesis in single living cells. Sci Rep. 2014;4:6807.
Rysman E, Brusselmans K, Scheys K, Timmermans L, Derua R, Munck S, et al. De novo lipogenesis protects cancer cells from free radicals and chemotherapeutics by promoting membrane lipid saturation. Cancer Res. 2010;70:8117–26.
Chajès V, Cambot M, Moreau K, Lenoir GM, Joulin V. Acetyl-CoA carboxylase α is essential to breast cancer cell survival. Cancer Res. 2006;66:5287–94.
Flavin R, Peluso S, Nguyen PL, Loda M. Fatty acid synthase as a potential therapeutic target in cancer. Future Oncol. 2010;6:551–62.
Migita T, Narita T, Nomura K, Miyagi E, Inazuka F, Matsuura M, et al. ATP citrate lyase: activation and therapeutic implications in non–small cell lung cancer. Cancer Res. 2008;68:8547–54.
Hu WL, Jin L, Xu A, Wang YF, Thorne RF, Zhang XD, et al. GUARDIN is a p53-responsive long non-coding RNA that is essential for genomic stability. Nat Cell Biol. 2018;20:492–502.
Yang F, Zhang H, Mei Y, Wu M. Reciprocal regulation of HIF-1α and LincRNA-p21 modulates the warburg effect. Mol Cell. 2014;53:88–100.
Wang P, Xue Y, Han Y, Lin L, Wu C, Xu S, et al. The STAT3-binding long noncoding RNA lnc-DC controls human dendritic cell differentiation. Science. 2014;344:310–3.
Lin A, Hu Q, Li C, Xing Z, Ma G, Wang C, et al. The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors. Nat Cell Biol. 2017;19:238.
Miao Y-R, Liu W, Zhang Q, Guo A-Y. lncRNASNP2: an updated database of functional SNPs and mutations in human and mouse lncRNAs. Nucleic Acids Res. 2017;46:D276–D80.
Li J, Han L, Roebuck P, Diao L, Liu L, Yuan Y, et al. TANRIC: an interactive open platform to explore the function of lncRNAs in cancer. Cancer Res. 2015;75:3728–37.
Travis WD, Brambilla E, Riely GJ. New pathologic classification of lung cancer: relevance for clinical practice and clinical trials. J Clin Oncol. 2013;31:992–1001.