Structural insights into protein folding, stability and activity using

HEWL X-ray crystallography biophysical characterization isotope effect perdeuterated lysozyme protein refolding protein structure structural biology

Journal

IUCrJ
ISSN: 2052-2525
Titre abrégé: IUCrJ
Pays: England
ID NLM: 101623101

Informations de publication

Date de publication:
01 May 2021
Historique:
received: 30 11 2020
accepted: 03 02 2021
entrez: 6 5 2021
pubmed: 7 5 2021
medline: 7 5 2021
Statut: epublish

Résumé

This structural and biophysical study exploited a method of perdeuterating hen egg-white lysozyme based on the expression of insoluble protein in

Identifiants

pubmed: 33953924
doi: 10.1107/S2052252521001299
pii: S2052252521001299
pmc: PMC8086161
doi:

Types de publication

Journal Article

Langues

eng

Pagination

372-386

Informations de copyright

© Ramos et al. 2021.

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Auteurs

Joao Ramos (J)

Life Sciences Group, Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
Partnership for Structural Biology (PSB), 71 Avenue des Martyrs, 38000 Grenoble, France.
Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.

Valerie Laux (V)

Life Sciences Group, Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
Partnership for Structural Biology (PSB), 71 Avenue des Martyrs, 38000 Grenoble, France.

Michael Haertlein (M)

Life Sciences Group, Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
Partnership for Structural Biology (PSB), 71 Avenue des Martyrs, 38000 Grenoble, France.

Elisabetta Boeri Erba (E)

Partnership for Structural Biology (PSB), 71 Avenue des Martyrs, 38000 Grenoble, France.
Institut de Biologie Structurale, Université de Grenoble Alpes, CEA, CNRS, 71 Avenue des Martyrs, 38000 Grenoble, France.

Katherine E McAuley (KE)

Diamond Light Source, Didcot OX11 0DE, United Kingdom.
Paul Scherrer Institute, Forschungsstrasse 111, 5232 Villigen, Switzerland.

V Trevor Forsyth (VT)

Life Sciences Group, Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
Partnership for Structural Biology (PSB), 71 Avenue des Martyrs, 38000 Grenoble, France.
Faculty of Natural Sciences, Keele University, Newcastle-under-Lyme ST5 5BG, United Kingdom.

Estelle Mossou (E)

Life Sciences Group, Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
Partnership for Structural Biology (PSB), 71 Avenue des Martyrs, 38000 Grenoble, France.
Faculty of Natural Sciences, Keele University, Newcastle-under-Lyme ST5 5BG, United Kingdom.

Sine Larsen (S)

Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark.

Annette E Langkilde (AE)

Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.

Classifications MeSH