Sperm chromatin integrity in a man with macrocephaly syndrome.
DNA fragmentation
embryo quality
fertilisation
protamine deficiency
residual histone
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
revised:
22
04
2021
received:
17
12
2020
accepted:
23
04
2021
pubmed:
8
5
2021
medline:
9
7
2021
entrez:
7
5
2021
Statut:
ppublish
Résumé
This study reports chromatin status and ICSI outcomes in a case of sperm macrocephaly syndrome(SMS), showing 100% of spermatozoa with abnormal morphology. Percentages of sperm DNA fragmentation for TUNEL (31.7% versus 6.5%), SCSA (33% versus 25%) assays, chromatin maturity tests, CMA3 (58% versus 29%) and aniline blue (63% versus 35%) staining were higher in case sample compared to the fertile sample. Artificial oocyte activation resulted in a similar fertilisation rate between case and control samples (71% versus 66.7%), but the case showed delayed embryo development on day 3 post-insemination. Unlike fertile case, no embryos reached the blastocyst stage. The result of this case study shows that macrocephaly is associated with reduced chromatin maturity and DNA integrity. Although both cases showed a similar chance for fertilisation through artificial chemical activation for only macrocephalic man, the developmental competency is jeopardised in such cases.
Substances chimiques
Chromatin
0
Types de publication
Case Reports
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14100Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
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