Resolving the Spatial and Cellular Architecture of Lung Adenocarcinoma by Multiregion Single-Cell Sequencing.
Journal
Cancer discovery
ISSN: 2159-8290
Titre abrégé: Cancer Discov
Pays: United States
ID NLM: 101561693
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
03
09
2020
revised:
26
02
2021
accepted:
30
04
2021
pubmed:
12
5
2021
medline:
15
3
2022
entrez:
11
5
2021
Statut:
ppublish
Résumé
Little is known of the geospatial architecture of individual cell populations in lung adenocarcinoma (LUAD) evolution. Here, we perform single-cell RNA sequencing of 186,916 cells from five early-stage LUADs and 14 multiregion normal lung tissues of defined spatial proximities from the tumors. We show that cellular lineages, states, and transcriptomic features geospatially evolve across normal regions to LUADs. LUADs also exhibit pronounced intratumor cell heterogeneity within single sites and transcriptional lineage-plasticity programs. T regulatory cell phenotypes are increased in normal tissues with proximity to LUAD, in contrast to diminished signatures and fractions of cytotoxic CD8
Identifiants
pubmed: 33972311
pii: 2159-8290.CD-20-1285
doi: 10.1158/2159-8290.CD-20-1285
pmc: PMC8487926
mid: NIHMS1702957
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2506-2523Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL130129
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA016672
Pays : United States
Organisme : NCI NIH HHS
ID : U2C CA233238
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA205608
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL153511
Pays : United States
Organisme : NIH HHS
ID : S10 OD024977
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA070907
Pays : United States
Organisme : CSRD VA
ID : I01 CX000345
Pays : United States
Informations de copyright
©2021 American Association for Cancer Research.
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