2'-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography.
Crystallography
Methylation
Methyltransferases
/ chemistry
Multiprotein Complexes
/ chemistry
RNA Cap Analogs
/ chemistry
RNA Caps
/ chemistry
RNA, Messenger
/ chemistry
RNA, Viral
/ chemistry
S-Adenosylhomocysteine
/ chemistry
S-Adenosylmethionine
/ chemistry
SARS-CoV-2
/ chemistry
Synchrotrons
Viral Nonstructural Proteins
/ chemistry
Viral Regulatory and Accessory Proteins
/ chemistry
CAP-1
Nsp10/16
SARS-CoV-2
mRNA
serial crystallography
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
25 05 2021
25 05 2021
Historique:
entrez:
11
5
2021
pubmed:
12
5
2021
medline:
20
5
2021
Statut:
ppublish
Résumé
The genome of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coronavirus has a capping modification at the 5'-untranslated region (UTR) to prevent its degradation by host nucleases. These modifications are performed by the Nsp10/14 and Nsp10/16 heterodimers using S-adenosylmethionine as the methyl donor. Nsp10/16 heterodimer is responsible for the methylation at the ribose 2'-O position of the first nucleotide. To investigate the conformational changes of the complex during 2'-O methyltransferase activity, we used a fixed-target serial synchrotron crystallography method at room temperature. We determined crystal structures of Nsp10/16 with substrates and products that revealed the states before and after methylation, occurring within the crystals during the experiments. Here we report the crystal structure of Nsp10/16 in complex with Cap-1 analog (
Identifiants
pubmed: 33972410
pii: 2100170118
doi: 10.1073/pnas.2100170118
pmc: PMC8166198
pii:
doi:
Substances chimiques
Multiprotein Complexes
0
NSP10 protein, SARS-CoV-2
0
NSP16 protein, SARS-CoV-2
0
RNA Cap Analogs
0
RNA Caps
0
RNA, Messenger
0
RNA, Viral
0
Viral Nonstructural Proteins
0
Viral Regulatory and Accessory Proteins
0
S-Adenosylmethionine
7LP2MPO46S
S-Adenosylhomocysteine
979-92-0
Methyltransferases
EC 2.1.1.-
cap I RNA (nucleoside-2'-)methyltransferase
EC 2.1.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 the Author(s). Published by PNAS.
Déclaration de conflit d'intérêts
The authors declare no competing interest.
Références
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W615-9
pubmed: 15215462
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
Methods Enzymol. 2003;374:229-44
pubmed: 14696376
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Nature. 2010 Nov 18;468(7322):452-6
pubmed: 21085181
J Mol Biol. 2002 Jul 5;320(2):223-35
pubmed: 12079381
Immunity. 2015 Jul 21;43(1):41-51
pubmed: 26187414
RNA Biol. 2014;11(12):1597-607
pubmed: 25626080
Acta Crystallogr D Struct Biol. 2017 Mar 1;73(Pt 3):223-233
pubmed: 28291757
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Sci Signal. 2020 Sep 29;13(651):
pubmed: 32994211
Chembiochem. 2012 Dec 21;13(18):2642-55
pubmed: 23180741
Nat Rev Microbiol. 2003 Dec;1(3):209-18
pubmed: 15035025
J Virol. 2017 Feb 14;91(5):
pubmed: 28031370
Nucleic Acids Res. 2018 Sep 6;46(15):7902-7912
pubmed: 30192980
Sci Signal. 2021 Jun 29;14(689):
pubmed: 34131072
Gene. 2003 Jan 2;302(1-2):129-38
pubmed: 12527203
Protein Eng. 2002 Feb;15(2):101-8
pubmed: 11917146
Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):596-601
pubmed: 26733676
Nat Commun. 2020 Jul 24;11(1):3717
pubmed: 32709887
J Biol Chem. 1978 Mar 10;253(5):1710-5
pubmed: 627567
Acta Crystallogr D Biol Crystallogr. 2008 Jan;64(Pt 1):33-9
pubmed: 18094465
Nat Immunol. 2011 Feb;12(2):137-43
pubmed: 21217758
Signal Transduct Target Ther. 2020 Jul 29;5(1):131
pubmed: 32728018
Lancet Infect Dis. 2020 May;20(5):533-534
pubmed: 32087114
Elife. 2015 Mar 17;4:
pubmed: 25781634
Eur J Biochem. 1997 Jul 15;247(2):461-9
pubmed: 9266685
Acta Crystallogr D Struct Biol. 2017 Apr 1;73(Pt 4):373-378
pubmed: 28375148
Virus Res. 2014 Dec 19;194:191-9
pubmed: 25278144
J Virol. 2008 Aug;82(16):8071-84
pubmed: 18417574
PLoS Pathog. 2011 May;7(5):e1002059
pubmed: 21637813
PLoS Pathog. 2010 Apr 22;6(4):e1000863
pubmed: 20421945
Sci Rep. 2019 May 6;9(1):6971
pubmed: 31061502
Cell Host Microbe. 2020 Mar 11;27(3):325-328
pubmed: 32035028
Lancet. 2020 Feb 15;395(10223):497-506
pubmed: 31986264
Nat Commun. 2020 Jul 24;11(1):3718
pubmed: 32709886
Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):7954-9
pubmed: 26080432
PLoS Pathog. 2011 Oct;7(10):e1002294
pubmed: 22022266
Acta Crystallogr D Struct Biol. 2018 Jun 1;74(Pt 6):506-518
pubmed: 29872002
Concurr Comput. 2015 Feb;27(2):290-305
pubmed: 25642152