Production and structure prediction of amylases from Chlorella vulgaris.
Bioinformatic tools
Culture mode
Enzymes
Glycoside hydrolase
Microalgae
Molecular modeling
Starch
Journal
Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
25
01
2021
accepted:
06
05
2021
pubmed:
12
5
2021
medline:
25
9
2021
entrez:
11
5
2021
Statut:
ppublish
Résumé
Amylases are enzymes required for starch degradation and are naturally produced by many microorganisms. These enzymes are used in several fields such as food processing, beverage, and medicine as well as in the formulation of enzymatic detergents proving their significance in modern biotechnology. In this study, a three-stage growth mode was applied to enhance starch production and amylase detection from Chlorella vulgaris. Stress conditions applied in the second stage of cultivation led to an accumulation of proteins (75% DW) and starch (21% DW) and a decrease in biomass. Amylase activities were detected and they showed high production levels especially on day 3 (35 U/ml) and day 5 (22.5 U/ml) of the second and third stages, respectively. The bioinformatic tools used to seek amylase protein sequences from TSA database of C. vulgaris revealed 7 putative genes encoding for 4 α-amylases, 2 β-amylases, and 1 isoamylase. An in silico investigation showed that these proteins are different in their lengths as well as in their cellular localizations and oligomeric states though they share common features like CSRs of GH13 family or active site of GH14 family. In brief, this study allowed for the production and in silico characterization of amylases from C. vulgaris.
Identifiants
pubmed: 33973124
doi: 10.1007/s11356-021-14357-9
pii: 10.1007/s11356-021-14357-9
doi:
Substances chimiques
Starch
9005-25-8
Amylases
EC 3.2.1.-
alpha-Amylases
EC 3.2.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
51046-51059Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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