Strain and host-cell dependent role of type-1 fimbriae in the adherence phenotype of super-shed Escherichia coli O157:H7.
Escherichia coli
FimH
O157:H7
Super-shedder
Type-1 fimbriae
Journal
International journal of medical microbiology : IJMM
ISSN: 1618-0607
Titre abrégé: Int J Med Microbiol
Pays: Germany
ID NLM: 100898849
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
05
01
2021
revised:
14
04
2021
accepted:
03
05
2021
pubmed:
12
5
2021
medline:
2
6
2021
entrez:
11
5
2021
Statut:
ppublish
Résumé
Super-shed (SS) Escherichia coli O157 (E. coli O157) demonstrate a strong, aggregative, locus of enterocyte effacement (LEE)-independent adherence phenotype on bovine recto-anal junction squamous epithelial (RSE) cells, and harbor polymorphisms in non-LEE-adherence-related loci, including in the type 1 fimbriae operon. To elucidate the role of type 1 fimbriae in strain- and host-specific adherence, we evaluated the entire Fim operon (FimB-H) and its adhesion (FimH) deletion mutants in four E. coli O157 strains, SS17, SS52, SS77 and EDL933, and evaluated the adherence phenotype in bovine RSE and human HEp-2 adherence assays. Consistent with the prevailing dogma that fimH expression is genetically switched off in E. coli O157, the ΔfimHSS52, ΔfimB-HSS52, ΔfimB-HSS17, and ΔfimHSS77 mutants remained unchanged in adherence phenotype to RSE cells. In contrast, the ΔfimHSS17 and ΔfimB-HSS77 mutants changed from a wild-type strong and aggregative, to a moderate and diffuse adherence phenotype, while both ΔfimHEDL933 and ΔfimB-HEDL933 mutants demonstrated enhanced binding to RSE cells (p < 0.05). Additionally, both ΔfimHSS17 and ΔfimHEDL933 were non-adherent to HEp-2 cells (p < 0.05). Complementation of the mutant strains with their respective wild-type genes restored parental phenotypes. Microscopy revealed that the SS17 and EDL933 strains indeed carry type 1 fimbriae-like structures shorter than those seen in uropathogenic E. coli. Taken together, these results provide compelling evidence for a strain and host cell type-dependent role of fimH and the fim operon in E. coli O157 adherence that needs to be further evaluated.
Identifiants
pubmed: 33975122
pii: S1438-4221(21)00040-0
doi: 10.1016/j.ijmm.2021.151511
pmc: PMC8605689
mid: NIHMS1705913
pii:
doi:
Substances chimiques
DNA-Binding Proteins
0
Escherichia coli Proteins
0
fimB protein, E coli
0
Integrases
EC 2.7.7.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
151511Subventions
Organisme : NIAID NIH HHS
ID : R21 AI147575
Pays : United States
Informations de copyright
Published by Elsevier GmbH.
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