Analysis of the Evolution of Pandemic Influenza A(H1N1) Virus Neuraminidase Reveals Entanglement of Different Phenotypic Characteristics.
Animals
Binding Sites
Cells, Cultured
Dogs
Epithelial Cells
/ virology
Evolution, Molecular
Female
HEK293 Cells
Hemagglutinin Glycoproteins, Influenza Virus
/ chemistry
Humans
Influenza A Virus, H1N1 Subtype
/ classification
Influenza, Human
/ virology
Madin Darby Canine Kidney Cells
Neuraminidase
/ chemistry
Pandemics
Phenotype
Phylogeny
Virion
antigenicity
enzymatic activity
influenza A virus
neuraminidase
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
11 05 2021
11 05 2021
Historique:
entrez:
12
5
2021
pubmed:
13
5
2021
medline:
16
11
2021
Statut:
epublish
Résumé
The influenza A virus (IAV) neuraminidase (NA) is essential for virion release from cells and decoy receptors and an important target of antiviral drugs and antibodies. Adaptation to a new host sialome and escape from the host immune system are forces driving the selection of mutations in the NA gene. Phylogenetic analysis shows that until 2015, 16 amino acid substitutions in NA became fixed in the virus population after introduction in the human population of the pandemic IAV H1N1 (H1N1pdm09) in 2009. The accumulative effect of these substitutions, in the order in which they appeared, was analyzed using recombinant proteins and viruses in combination with different functional assays. The results indicate that NA activity did not evolve to a single optimum but rather fluctuated within a certain bandwidth. Furthermore, antigenic and enzymatic properties of NA were intertwined, with several residues affecting multiple properties. For example, the substitution K432E in the second sialic acid binding site, next to the catalytic site, was shown to affect catalytic activity, substrate specificity, and the pH optimum for maximum activity. This substitution also altered antigenicity of NA, which may explain its selection. We propose that the entanglement of NA phenotypes may be an important determining factor in the evolution of NA.
Identifiants
pubmed: 33975931
pii: mBio.00287-21
doi: 10.1128/mBio.00287-21
pmc: PMC8262965
pii:
doi:
Substances chimiques
Hemagglutinin Glycoproteins, Influenza Virus
0
Neuraminidase
EC 3.2.1.18
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : HHSN272201400008C
Pays : United States
Informations de copyright
Copyright © 2021 Dai et al.
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