Facile isolation and analysis of sporopollenin exine from bee pollen.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 05 2021
11 05 2021
Historique:
received:
03
08
2019
accepted:
24
03
2021
entrez:
12
5
2021
pubmed:
13
5
2021
medline:
9
11
2021
Statut:
epublish
Résumé
We present facile methods to obtain purified sporopollenin exine capsules, and provide mass balances for classical and novel purification procedures. An ionic liquid, tetrabutyl phosphonium hydroxide turned out to be the most effective in removing the intine wall. The sporopollenin capsules were investigated by fluorescent microscopy, AFM, solid-state NMR and infrared Raman spectroscopy. The latter two methods showed that sunflower and rape exines have different proportions of O-aliphatic and aromatic constituents. Purified exine capsules were coated with functionalized fluorophores. The procedures presented in this paper could contribute to further spread of the applications of this hollow, and chemically highly resistant material.
Identifiants
pubmed: 33976296
doi: 10.1038/s41598-021-87619-8
pii: 10.1038/s41598-021-87619-8
pmc: PMC8113464
doi:
Substances chimiques
Biopolymers
0
Capsules
0
Organophosphorus Compounds
0
sporopollenin
12712-72-0
Carotenoids
36-88-4
tetrabutylphosphonium
NIJ70MFW91
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9952Références
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