Analysis of waveform and amplitude of mouse rod and cone flash responses.
cone
mathematical model
photoreceptor
retina
rod
vision
Journal
The Journal of physiology
ISSN: 1469-7793
Titre abrégé: J Physiol
Pays: England
ID NLM: 0266262
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
11
12
2020
accepted:
28
04
2021
pubmed:
13
5
2021
medline:
11
8
2021
entrez:
12
5
2021
Statut:
ppublish
Résumé
Most vertebrate eyes have rod and cone photoreceptors, which use a signal transduction pathway consisting of many biological processes to transform light into an electrical response. We dissect and quantify the contribution of each of these processes to the photoreceptor light response by using a novel method of analysis that provides an analytical solution for the entire time course of the dim-flash light response. We find that the shape of the light response is exclusively controlled by deactivation parameters. Activation parameters scale this shape and alter the response amplitude. We show that the rising phase of the response depends on Ca Vertebrate eyes have rod and cone photoreceptors, which use a complex transduction pathway comprising many biological processes to transform the absorption of light into an electrical response. A fundamental question in sensory transduction is how these processes contribute to the response. To study this question, we use a well-accepted phototransduction model, which we analyse with a novel method based on the log transform of the current. We derive an analytical solution that describes the entire time course of the photoreceptor response to dim flashes of light. We use this solution to dissect and quantify the contribution of each process to the response. We find that the entire dim-flash response is proportional to the flash intensity. By normalizing responses to unit amplitude, we define a waveform that is independent of the light intensity and characterizes the invariant shape of dim-flash responses. We show that this waveform is exclusively determined by deactivation rates; activation rates only scale the waveform and affect the amplitude. This analysis corrects a previous assumption that the rising phase is determined entirely by activation rates. We further show that the rising phase depends on Ca
Identifiants
pubmed: 33977528
doi: 10.1113/JP281225
pmc: PMC8259453
mid: NIHMS1703510
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3295-3312Subventions
Organisme : NEI NIH HHS
ID : R01 EY001844
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY003311
Pays : United States
Organisme : NEI NIH HHS
ID : R37 EY001844
Pays : United States
Informations de copyright
© 2021 The Authors. The Journal of Physiology © 2021 The Physiological Society.
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