Human DICER helicase domain recruits PKR and modulates its antiviral activity.
Alphavirus Infections
/ drug therapy
Antiviral Agents
/ pharmacology
DEAD-box RNA Helicases
/ genetics
HEK293 Cells
Humans
Interferon Type I
/ pharmacology
Protein Interaction Domains and Motifs
/ drug effects
Ribonuclease III
/ genetics
Semliki forest virus
/ drug effects
Virus Replication
eIF-2 Kinase
/ genetics
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
02
12
2020
accepted:
08
04
2021
entrez:
13
5
2021
pubmed:
14
5
2021
medline:
5
10
2021
Statut:
epublish
Résumé
The antiviral innate immune response mainly involves type I interferon (IFN) in mammalian cells. The contribution of the RNA silencing machinery remains to be established, but several recent studies indicate that the ribonuclease DICER can generate viral siRNAs in specific conditions. It has also been proposed that type I IFN and RNA silencing could be mutually exclusive antiviral responses. In order to decipher the implication of DICER during infection of human cells with alphaviruses such as the Sindbis virus and Semliki forest virus, we determined its interactome by proteomics analysis. We show that DICER specifically interacts with several double-stranded RNA binding proteins and RNA helicases during viral infection. In particular, proteins such as DHX9, ADAR-1 and the protein kinase RNA-activated (PKR) are enriched with DICER in virus-infected cells. We demonstrate that the helicase domain of DICER is essential for this interaction and that its deletion confers antiviral properties to this protein in an RNAi-independent, PKR-dependent, manner.
Identifiants
pubmed: 33984068
doi: 10.1371/journal.ppat.1009549
pii: PPATHOGENS-D-20-02587
pmc: PMC8118307
doi:
Substances chimiques
Antiviral Agents
0
Interferon Type I
0
EIF2AK2 protein, human
EC 2.7.11.1
eIF-2 Kinase
EC 2.7.11.1
DICER1 protein, human
EC 3.1.26.3
Ribonuclease III
EC 3.1.26.3
DEAD-box RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009549Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
EMBO J. 2019 Apr 15;38(8):
pubmed: 30872283
Cell Rep. 2015 Apr 14;11(2):295-307
pubmed: 25843719
Biochemistry. 2003 Sep 2;42(34):10182-90
pubmed: 12939146
Viral Immunol. 2002;15(1):53-76
pubmed: 11952147
Curr Opin Struct Biol. 2010 Jun;20(3):313-24
pubmed: 20456941
Nature. 2011 Apr 28;472(7344):481-5
pubmed: 21478870
J Biol Chem. 2007 Jun 15;282(24):17649-57
pubmed: 17452327
Mol Cell. 2007 May 25;26(4):523-37
pubmed: 17531811
Biochemistry. 2011 May 31;50(21):4550-60
pubmed: 21526770
Nature. 2001 Jan 18;409(6818):363-6
pubmed: 11201747
Mol Cell. 2004 Jul 23;15(2):185-97
pubmed: 15260970
Int J Biochem Cell Biol. 1997 Jul;29(7):945-9
pubmed: 9375375
Nat Struct Mol Biol. 2006 Jan;13(1):13-21
pubmed: 16369484
Retrovirology. 2013 Sep 11;10:96
pubmed: 24020926
Viruses. 2016 Jun 11;8(6):
pubmed: 27294951
Genes Dev. 2014 Jun 15;28(12):1310-22
pubmed: 24939934
Sci Rep. 2019 Sep 24;9(1):13752
pubmed: 31551491
Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):709-14
pubmed: 9892698
Nucleic Acids Res. 2018 Sep 28;46(17):9134-9147
pubmed: 29939295
Science. 2013 Oct 11;342(6155):235-8
pubmed: 24115438
RNA. 2014 Jun;20(6):923-37
pubmed: 24757167
BMC Bioinformatics. 2013 Apr 15;14:128
pubmed: 23586463
J Immunol. 2017 Sep 1;199(5):1846-1855
pubmed: 28760879
Eur J Biochem. 1976 Jan 15;61(2):563-72
pubmed: 813998
J Biol Chem. 2006 May 5;281(18):12625-35
pubmed: 16527808
J Biol Chem. 2000 Dec 1;275(48):37993-8
pubmed: 10988289
PLoS Pathog. 2009 Feb;5(2):e1000311
pubmed: 19229320
Cell Rep. 2020 Feb 11;30(6):1690-1701.e4
pubmed: 32049003
Cell Rep. 2014 Jul 10;8(1):114-25
pubmed: 24953656
Oncogene. 1999 Nov 1;18(45):6112-20
pubmed: 10557102
Nucleic Acids Res. 2008 Feb;36(3):998-1008
pubmed: 18096616
EMBO J. 2016 Dec 1;35(23):2505-2518
pubmed: 27815315
Sci Rep. 2015 May 29;5:10693
pubmed: 26024431
Curr Biol. 2005 Dec 6;15(23):2149-55
pubmed: 16289642
Cell Host Microbe. 2013 Oct 16;14(4):374-8
pubmed: 24139396
Nat Rev Immunol. 2019 Jan;19(1):31-44
pubmed: 30301972
Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):E6945-54
pubmed: 26621737
J Virol. 2014 Jul;88(14):8065-76
pubmed: 24807715
EMBO J. 2006 Feb 8;25(3):522-32
pubmed: 16424907
Nat Microbiol. 2016 Dec 05;2:16250
pubmed: 27918527
EMBO J. 2018 Feb 15;37(4):
pubmed: 29351913
Nature. 2009 Jan 22;457(7228):485-9
pubmed: 19043403
Cell. 2013 Apr 25;153(3):575-89
pubmed: 23622242
RNA. 2013 Sep;19(9):1238-52
pubmed: 23882114
Nucleic Acids Res. 2018 Jul 2;46(W1):W242-W245
pubmed: 29762716
Proc Natl Acad Sci U S A. 1994 May 24;91(11):4713-7
pubmed: 7515177
mBio. 2013 Nov 19;4(6):e00698-13
pubmed: 24255120
Cell. 2018 Mar 22;173(1):20-51
pubmed: 29570994
J Biol Chem. 2001 Feb 23;276(8):5445-51
pubmed: 11096080
Nature. 2017 Apr 6;544(7648):115-119
pubmed: 28355180
Mol Cell Biol. 2009 Jan;29(1):254-65
pubmed: 18936160
Cell Host Microbe. 2016 Feb 10;19(2):142-9
pubmed: 26867173
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
Cell Host Microbe. 2011 Apr 21;9(4):299-309
pubmed: 21501829
Sci Adv. 2020 Feb 05;6(6):eaax7989
pubmed: 32076641
Nat Immunol. 2006 Jun;7(6):563-7
pubmed: 16715068
Front Mol Neurosci. 2019 Jan 09;11:480
pubmed: 30686999
Immunity. 2017 Jun 20;46(6):992-1004.e5
pubmed: 28636969
Genes (Basel). 2018 Oct 19;9(10):
pubmed: 30347765
Biochimie. 2007 Jun-Jul;89(6-7):799-811
pubmed: 17451862
Science. 2013 Oct 11;342(6155):231-4
pubmed: 24115437
Nature. 2005 Aug 4;436(7051):740-4
pubmed: 15973356
Virology. 2011 Mar 15;411(2):180-93
pubmed: 21211811
Wiley Interdiscip Rev RNA. 2015 May-Jun;6(3):271-89
pubmed: 25630541
J Virol. 2020 Apr 16;94(9):
pubmed: 32102877
Nat Rev Immunol. 2014 Jan;14(1):36-49
pubmed: 24362405
Trends Biochem Sci. 2015 Oct;40(10):576-585
pubmed: 26410598
PLoS One. 2013 Dec 31;8(12):e84070
pubmed: 24391886
Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8421-6
pubmed: 9237992
Int J Mol Sci. 2020 Feb 17;21(4):
pubmed: 32079277
Nucleic Acids Res. 2016 Jul 8;44(W1):W90-7
pubmed: 27141961
Immunity. 2017 Jun 20;46(6):970-972
pubmed: 28636964
J Biol Chem. 2010 Oct 22;285(43):33358-33370
pubmed: 20716523
Curr Opin Virol. 2018 Oct;32:9-14
pubmed: 30015014
Virology. 2010 Jan 20;396(2):316-22
pubmed: 19913273
J Mol Biol. 2008 Aug 29;381(2):351-60
pubmed: 18599071
EMBO Rep. 2005 Oct;6(10):961-7
pubmed: 16142218
Noncoding RNA. 2016 Oct 25;2(4):
pubmed: 29657270
Nat Rev Drug Discov. 2007 Dec;6(12):975-90
pubmed: 18049472
Curr Opin Immunol. 2011 Oct;23(5):573-82
pubmed: 21924887
Nat Genet. 2003 Nov;35(3):217-8
pubmed: 14528306
RNA. 2018 Sep;24(9):1172-1182
pubmed: 29903832
J Biomol Screen. 2016 Dec;21(10):1100-1111
pubmed: 27455993
PLoS Pathog. 2010 Feb 12;6(2):e1000764
pubmed: 20169186
J Virol. 2009 Oct;83(19):10119-28
pubmed: 19605474
Plant J. 2018 Oct;96(1):119-132
pubmed: 29983000
Nature. 2004 Sep 16;431(7006):343-9
pubmed: 15372041
Science. 2001 Aug 3;293(5531):834-8
pubmed: 11452083
BMC Mol Biol. 2009 May 07;10:38
pubmed: 19422693