Genomic investigation of a dengue virus outbreak in Thiès, Senegal, in 2018.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
14 05 2021
Historique:
received: 19 11 2020
accepted: 16 03 2021
entrez: 15 5 2021
pubmed: 16 5 2021
medline: 30 10 2021
Statut: epublish

Résumé

Dengue virus is a major and rapidly growing public health concern in tropic and subtropic regions across the globe. In late 2018, Senegal experienced its largest dengue virus outbreak to date, covering several regions. However, little is known about the genetic diversity of dengue virus (DENV) in Senegal. Here we report complete viral genomes from 17 previously undetected DENV cases from the city of Thiès. In total we identified 19 cases of DENV in a cohort of 198 individuals with fever collected in October and November 2018. We detected 3 co-circulating serotypes; DENV 3 was the most frequent accounting for 11/17 sequences (65%), 4 (23%) were DENV2 and 2 (12%) were DENV1. Sequences were most similar to recent sequences from West Africa, suggesting ongoing local circulation of viral populations; however, detailed inference is limited by the scarcity of available genomic data. We did not find clear associations with reported clinical signs or symptoms, highlighting the importance of testing for diagnosing febrile diseases. Overall, these findings expand the known range of DENV in Senegal, and underscore the need for better genomic characterization of DENV in West Africa.

Identifiants

pubmed: 33990632
doi: 10.1038/s41598-021-89070-1
pii: 10.1038/s41598-021-89070-1
pmc: PMC8121849
doi:

Substances chimiques

DNA, Viral 0

Types de publication

Journal Article Observational Study Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

10321

Subventions

Organisme : NIAID NIH HHS
ID : U01 AI151812
Pays : United States
Organisme : NHGRI NIH HHS
ID : U54 HG007480
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States

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Auteurs

Amy Gaye (A)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Tolla Ndiaye (T)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Mouhamad Sy (M)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Awa B Deme (AB)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Alphonse B Thiaw (AB)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Aita Sene (A)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Cheikh Ndiaye (C)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Younouss Diedhiou (Y)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Amadou M Mbaye (AM)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Ibrahima Ndiaye (I)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Christopher Tomkins-Tinch (C)

Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Department of Organismal and Evolutionary Biology, Harvard University, Cambridge, MA, USA.

Jules F Gomis (JF)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Aida S Badiane (AS)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Bronwyn MacInnis (B)

Broad Institute of MIT and Harvard, Cambridge, MA, USA.

Daniel J Park (DJ)

Broad Institute of MIT and Harvard, Cambridge, MA, USA.

Mouhamadou Ndiaye (M)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal.

Ngayo Sy (N)

Service de Lutte AntiParasitaire, Thiès, Senegal.

Pardis C Sabeti (PC)

Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Department of Organismal and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, MA, USA.
Howard Hughes Medical Institute (HHMI), Chevy Chase, MD, USA.

Katherine J Siddle (KJ)

Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Department of Organismal and Evolutionary Biology, Harvard University, Cambridge, MA, USA.

Daouda Ndiaye (D)

Laboratoire de Parasitologie-Mycologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop de Dakar, BP 16477, Dakar, Senegal. daouda.ndiaye@ucad.edu.sn.

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Classifications MeSH