Characterization of recombinant E. coli expressing a novel fucosidase from Bacillus cereus 2-8 belonging to GH95 family.

Bacillus cereus / enzymology Bacterial Proteins / chemistry Escherichia coli / genetics Hydrogen-Ion Concentration Recombinant Fusion Proteins / chemistry Temperature alpha-L-Fucosidase / chemistry
Bacillus cereus Fucoidan Fucosidase Function domain analysis

Journal

Protein expression and purification
ISSN: 1096-0279
Titre abrégé: Protein Expr Purif
Pays: United States
ID NLM: 9101496

Informations de publication

Date de publication:
10 2021
Historique:
received: 02 03 2020
revised: 09 03 2021
accepted: 30 04 2021
pubmed: 16 5 2021
medline: 29 1 2022
entrez: 15 5 2021
Statut: ppublish

Résumé

Fucoidan oligosaccharides possesses diverse physicochemical and biological activities. Specific glycoside hydrolases are valuable tools for degrading polysaccharides to produce oligosaccharides. In this study, BcFucA, a novel fucosidase belonging to GH95 family from Bacillus cereus 2-8, was cloned into pET21a vector, expressed in E. coli BL21 (DE3) and characterized. The protein consists of 1136 amino acid residues encoded by 3411 bases and has a molecular weight of 125.35 kDa. The optimal temperature and pH of this enzyme are 50 °C and pH 4.0. In addition, this study showed that the unknown function domain (encoding Lys261-Thr681) defined as a linker is quite important for its activity. The obtained novel enzyme BcFucA will contribute to the effective degradation of fucoidan and future industrial applications.

Identifiants

DOI: 10.1016/j.pep.2021.105897 PMID: 33991676
pubmed: 33991676
pii: S1046-5928(21)00080-2
doi: 10.1016/j.pep.2021.105897
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Recombinant Fusion Proteins 0
alpha-L-Fucosidase EC 3.2.1.51

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

105897

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Qian Li (Q)

Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian, 116023, China; Department of Food Science and Engineering, Dalian Ocean University, Dalian, 116023, China.

Chaofeng Jiang (C)

Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Haidong Tan (H)

Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian, 116023, China.

Xiaoming Zhao (X)

Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian, 116023, China.

Kuikui Li (K)

Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian, 116023, China. Electronic address: lkk@dicp.ac.cn.

Heng Yin (H)

Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian, 116023, China. Electronic address: yinheng@dicp.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus cereus › Characterization of recombinant E. coli...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Characterization of recombinant E. coli...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Characterization of recombinant E. coli...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Characterization of recombinant E. coli...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Protéines de fusion recombinantes › Characterization of recombinant E. coli...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Characterization of recombinant E. coli...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › alpha-L-Fucosidase › Characterization of recombinant E. coli...