Interspecies cell fusion between mouse embryonic stem cell and porcine pluripotent cell.
hybrid cells
interspecies cell fusion
mouse
pig
pluripotency
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
12
04
2021
accepted:
12
05
2021
pubmed:
17
5
2021
medline:
27
10
2021
entrez:
16
5
2021
Statut:
ppublish
Résumé
In the area of stem cell research, fusion of somatic cells into pluripotent cells such as mouse embryonic stem (ES) cells induces reprogramming of the somatic nucleus and can be used to study the effect of trans-acting factors from the pluripotent cell on the pluripotent state of somatic nucleus. As many other groups, we previously established a porcine pluripotent cell line at a low potential. Therefore, here, we performed experiments to investigate if the fusion with mouse ES cell could improve the pluripotent state of porcine pluripotent cell. Our data showed that resultant mouse-porcine interspecies fused cells are AP positive, and could be passaged up to 20 passages. Different degrees of increases in expression of porcine pluripotent genes proved that pig-origin gene network can be programmed by mouse ES. Further differentiation study also confirmed these fused cells' potential to form three germ layers. However, unexpectedly, we found that chromosome loss and aberrant (especially in porcine chromosomes) is severe after the cell fusion, implying that interspecies cell fusion may be not suitable to study porcine pluripotency without additional supportive conditions for genome stabilization.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1095-1103Subventions
Organisme : Inner Mongolia Agricultural University Grant
ID : NDYB2016-01
Organisme : Inner Mongolia Agricultural University Grant
ID : QN202004
Informations de copyright
© 2021 Wiley-VCH GmbH.
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