Transforming eukaryotic cell culture with macromolecular crowding.

Eukaryotic Cells Extracellular Matrix Macromolecular Substances
cell culture drug discovery excluded volume effect extracellular matrix macromolecular crowding regenerative medicine

Journal

Trends in biochemical sciences
ISSN: 0968-0004
Titre abrégé: Trends Biochem Sci
Pays: England
ID NLM: 7610674

Informations de publication

Date de publication:
10 2021
Historique:
received: 18 01 2021
revised: 07 04 2021
accepted: 16 04 2021
pubmed: 18 5 2021
medline: 28 9 2021
entrez: 17 5 2021
Statut: ppublish

Résumé

In multicellular organisms, the intracellular and extracellular spaces are considerably packed with a diverse range of macromolecular species. Yet, standard eukaryotic cell culture is performed in dilute, and deprived of macromolecules culture media, that barely imitate the density and complex macromolecular composition of tissues. Essentially, we drown cells in a sea of media and then expect them to perform physiologically. Herein, we argue the use of macromolecular crowding (MMC) in eukaryotic cell culture for regenerative medicine and drug discovery purposes.

Identifiants

DOI: 10.1016/j.tibs.2021.04.006 PMID: 33994289
pubmed: 33994289
pii: S0968-0004(21)00074-8
doi: 10.1016/j.tibs.2021.04.006
pii:
doi:

Substances chimiques

Macromolecular Substances 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

805-811

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no conflicts of interest.

Auteurs

Michael Raghunath (M)

Center for Cell Biology and Tissue Engineering, Institute for Chemistry and Biotechnology, Zurich University of Applied Sciences, Wädenswil, Switzerland.

Dimitrios I Zeugolis (DI)

Regenerative, Modular, and Developmental Engineering Laboratory (REMODEL), National University of Ireland Galway (NUI Galway), Galway, Ireland; Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway (NUI Galway), Galway, Ireland; Regenerative, Modular, and Developmental Engineering Laboratory (REMODEL), Faculty of Biomedical Sciences, Università della Svizzera Italiana (USI), Lugano, Switzerland; Regenerative, Modular, and Developmental Engineering Laboratory (REMODEL), School of Mechanical and Materials Engineering, University College Dublin (UCD), Dublin, Ireland. Electronic address: dimitrios.zevgolis@ucd.ie.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules eucaryotes › Transforming eukaryotic cell culture with...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Matrice extracellulaire › Transforming eukaryotic cell culture with...
questionsmedicales.fr › Structures macromoléculaires › Transforming eukaryotic cell culture with...