A novel mast cell-dependent allergic peritonitis model.
Animals
Asthma
/ immunology
Dermatitis, Atopic
/ immunology
Disease Models, Animal
Enterotoxins
/ immunology
Female
Immunization
/ methods
Immunoglobulin E
/ blood
Inflammation
/ immunology
Male
Mast Cells
/ immunology
Mice
Mice, Inbred C57BL
Ovalbumin
/ immunology
Peritonitis
/ immunology
Staphylococcus aureus
/ metabolism
allergic inflammation
allergic peritonitis
animal models
eosinophils
mast cells
Journal
Clinical and experimental immunology
ISSN: 1365-2249
Titre abrégé: Clin Exp Immunol
Pays: England
ID NLM: 0057202
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
revised:
04
04
2021
received:
20
01
2021
accepted:
03
05
2021
pubmed:
18
5
2021
medline:
29
12
2021
entrez:
17
5
2021
Statut:
ppublish
Résumé
Typical murine models of allergic inflammation are induced by the combination of ovalbumin and aluminum hydroxide. However, accumulating evidence indicates that, in models of asthma and atopic dermatitis, allergic inflammation can be generated in the absence of aluminum hydroxide. Moreover, co-administration of Staphylococcus aureus enterotoxin B with ovalbumin can enhance inflammation. The objective of this study was to establish a rapid and mast cell-dependent murine model of allergic inflammation by inducing allergic peritonitis using ovalbumin and S. aureus enterotoxin B. Allergic peritonitis was induced in C57BL/6 mice by subcutaneous sensitization and intraperitoneal challenge with ovalbumin and S. aureus enterotoxin B. Disease characteristics were assessed by flow cytometry, enzyme-linked immunosorbent assay (ELISA), trypan blue exclusion and colorimetric assays. The time-course of the allergic peritonitis revealed a peak of peritoneal inflammation 48 h after challenge, as assessed by total cells and eosinophil counts. The decrease of cell numbers started 96 h post-challenge, with complete clearance within 168 h. Moreover, significantly higher levels of tryptase and increased vascular permeability were found 30 min following challenge. Allergic inflammation induction by ovalbumin and S. aureus enterotoxin B was impaired in mast cell-deficient mice and partially restored by mice reconstitution with bone marrow-derived mast cells, indicating the mast cell role in this model. We present a novel model of allergic peritonitis that is mast cell-dependent, simple and robust. Moreover, the use of S. aureus enterotoxin B better resembles human allergic inflammation, which is known to be characterized by the colonization of S. aureus.
Identifiants
pubmed: 33999404
doi: 10.1111/cei.13619
pmc: PMC8374212
doi:
Substances chimiques
Enterotoxins
0
Immunoglobulin E
37341-29-0
enterotoxin B, staphylococcal
39424-53-8
Ovalbumin
9006-59-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
306-315Subventions
Organisme : Vigevani Foundation
Organisme : Rosetrees Trust
Organisme : Israel Science Foundation
ID : 472/15
Organisme : Aimwell Trust
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2021 British Society for Immunology.
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