Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production
Cancer
Flow Cytometry/Mass Cytometry
Immunology
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 06 2021
18 06 2021
Historique:
entrez:
20
5
2021
pubmed:
21
5
2021
medline:
21
5
2021
Statut:
epublish
Résumé
Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor. Here, we describe a protocol to evaluate the influence of HCQ on lymphocyte function by measuring the in vitro and ex vivo proliferation and cytokine production. The protocol can provide insights into potential immunomodulatory effects of HCQ and can be used for assessing other medications' effects on lymphocyte functions. For complete details on the use and execution of this protocol, please refer to Wabitsch et al. (2021).
Identifiants
pubmed: 34013212
doi: 10.1016/j.xpro.2021.100517
pii: S2666-1667(21)00224-0
pmc: PMC8113974
doi:
Substances chimiques
Cytokines
0
Immunologic Factors
0
Hydroxychloroquine
4QWG6N8QKH
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100517Subventions
Organisme : Intramural NIH HHS
ID : ZIA BC011345
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA BC010870
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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