Mass Cytometry, Imaging Mass Cytometry, and Multiplexed Ion Beam Imaging Use in a Clinical Setting.

Antibodies Diagnostic Imaging Flow Cytometry Image Cytometry Mass Spectrometry
High-parameter data analysis Imaging mass cytometry Mass cytometry Mass spectroscopy time of flight Multiplex ion beam imaging

Journal

Clinics in laboratory medicine
ISSN: 1557-9832
Titre abrégé: Clin Lab Med
Pays: United States
ID NLM: 8100174

Informations de publication

Date de publication:
06 2021
Historique:
entrez: 22 5 2021
pubmed: 23 5 2021
medline: 26 10 2021
Statut: ppublish

Résumé

Mass cytometry (MC), imaging mass cytometry (IMC), and multiplexed ion beam imaging (MIBI) represent a new generation of tools to understand increasingly complex systems. Although these technologies differ in their intended applications, with MC being most similar to flow cytometry, and IMC/MIBI being similar to immunohistochemistry, they all share a time of flight mass spectrometry (TOF MS) platform. These TOF MS platforms use metal conjugated antibodies as opposed to fluorophores, increasing the measurable parameters up to approximately 50 with a theoretic limit approximately 100 parameters. These tools are being adapted to understand highly complex systems in basic and clinical research.

Identifiants

DOI: 10.1016/j.cll.2021.03.008 PMID: 34020765
pubmed: 34020765
pii: S0272-2712(21)00008-1
doi: 10.1016/j.cll.2021.03.008
pii:
doi:

Substances chimiques

Antibodies 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

297-308

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Disclosure The authors declare they have no conflict of interests.

Auteurs

Raymond D Devine (RD)

Division of Hematology, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Comprehensive Cancer Center, Columbus, OH, USA.

Gregory K Behbehani (GK)

Division of Hematology, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Comprehensive Cancer Center, Columbus, OH, USA. Electronic address: Gregory.Behbehani@OSUMC.edu.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Mass Cytometry, Imaging Mass Cytometry, and...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Imagerie diagnostique › Mass Cytometry, Imaging Mass Cytometry, and...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › Mass Cytometry, Imaging Mass Cytometry, and...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en images › Mass Cytometry, Imaging Mass Cytometry, and...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Mass Cytometry, Imaging Mass Cytometry, and...