Inoculum effect of antimicrobial peptides.
Anti-Bacterial Agents
/ chemistry
Antimicrobial Peptides
/ chemistry
Bacteria
/ drug effects
Bacterial Infections
/ drug therapy
Bacterial Load
/ drug effects
Cell Membrane
/ drug effects
Drug Synergism
Humans
Microbial Sensitivity Tests
Peptidomimetics
/ pharmacology
Staphylococcus aureus
/ drug effects
Stereoisomerism
antimicrobial activity
antimicrobial peptides
inoculum effect
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
25 05 2021
25 05 2021
Historique:
entrez:
22
5
2021
pubmed:
23
5
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
The activity of many antibiotics depends on the initial density of cells used in bacterial growth inhibition assays. This phenomenon, termed the inoculum effect, can have important consequences for the therapeutic efficacy of the drugs, because bacterial loads vary by several orders of magnitude in clinically relevant infections. Antimicrobial peptides are a promising class of molecules in the fight against drug-resistant bacteria because they act mainly by perturbing the cell membranes rather than by inhibiting intracellular targets. Here, we report a systematic characterization of the inoculum effect for this class of antibacterial compounds. Minimum inhibitory concentration values were measured for 13 peptides (including all-D enantiomers) and peptidomimetics, covering more than seven orders of magnitude in inoculated cell density. In most cases, the inoculum effect was significant for cell densities above the standard inoculum of 5 × 10
Identifiants
pubmed: 34021080
pii: 2014364118
doi: 10.1073/pnas.2014364118
pmc: PMC8166072
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Antimicrobial Peptides
0
Peptidomimetics
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Déclaration de conflit d'intérêts
The authors declare no competing interest.
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