2,6-diaminopurine promotes repair of DNA lesions under prebiotic conditions.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
21 05 2021
Historique:
received: 16 06 2020
accepted: 20 04 2021
entrez: 22 5 2021
pubmed: 23 5 2021
medline: 12 6 2021
Statut: epublish

Résumé

High-yielding and selective prebiotic syntheses of RNA and DNA nucleotides involve UV irradiation to promote the key reaction steps and eradicate biologically irrelevant isomers. While these syntheses were likely enabled by UV-rich prebiotic environment, UV-induced formation of photodamages in polymeric nucleic acids, such as cyclobutane pyrimidine dimers (CPDs), remains the key unresolved issue for the origins of RNA and DNA on Earth. Here, we demonstrate that substitution of adenine with 2,6-diaminopurine enables repair of CPDs with yields reaching 92%. This substantial self-repairing activity originates from excellent electron donating properties of 2,6-diaminopurine in nucleic acid strands. We also show that the deoxyribonucleosides of 2,6-diaminopurine and adenine can be formed under the same prebiotic conditions. Considering that 2,6-diaminopurine was previously shown to increase the rate of nonenzymatic RNA replication, this nucleobase could have played critical roles in the formation of functional and photostable RNA/DNA oligomers in UV-rich prebiotic environments.

Identifiants

pubmed: 34021158
doi: 10.1038/s41467-021-23300-y
pii: 10.1038/s41467-021-23300-y
pmc: PMC8139960
doi:

Substances chimiques

Nucleic Acids 0
Nucleotides 0
Pyrimidine Dimers 0
2-Aminopurine 452-06-2
2,6-diaminopurine 49P95BAU4Z
RNA 63231-63-0
DNA 9007-49-2
Adenine JAC85A2161

Banques de données

figshare
['10.6084/m9.figshare.14268056']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3018

Subventions

Organisme : Medical Research Council
ID : MC_UP_A024_1009
Pays : United Kingdom

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Auteurs

Rafał Szabla (R)

EaStCHEM School of Chemistry, University of Edinburgh, Edinburgh, UK. rafal.szabla@ed.ac.uk.
Institute of Physics, Polish Academy of Sciences, Warsaw, Poland. rafal.szabla@ed.ac.uk.

Magdalena Zdrowowicz (M)

Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland. magdalena.zdrowowicz@ug.edu.pl.

Paulina Spisz (P)

Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland.

Nicholas J Green (NJ)

MRC Laboratory of Molecular Biology, Cambridge, UK.

Petr Stadlbauer (P)

Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic.

Holger Kruse (H)

Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic.

Jiří Šponer (J)

Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic.

Janusz Rak (J)

Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland.

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Classifications MeSH