Persistence of Zika virus RNA in the epididymis of the murine male reproductive tract.
Animals
Cell Line
Chlorocebus aethiops
Cyclophosphamide
/ pharmacology
Epididymis
/ virology
Immune Tolerance
/ immunology
Immunocompromised Host
/ immunology
Immunosuppressive Agents
/ pharmacology
Male
Mice
Mice, Inbred C57BL
Persistent Infection
/ virology
RNA, Viral
/ genetics
Recurrence
Semen
/ virology
Sexually Transmitted Diseases, Viral
/ transmission
Vero Cells
Virus Shedding
/ genetics
Zika Virus
/ genetics
Zika Virus Infection
/ transmission
Epididymis
Male reproductive tract
Mouse model
Recrudescence
Semen
Sexual transmission
Zika virus
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
received:
05
02
2021
revised:
06
04
2021
accepted:
03
05
2021
pubmed:
24
5
2021
medline:
7
1
2022
entrez:
23
5
2021
Statut:
ppublish
Résumé
Zika virus (ZIKV) can infect developing fetuses in utero and cause severe congenital defects independent of route of maternal infection. Infected men can shed ZIKV RNA in semen for over six months. Whether prolonged viral RNA shedding in semen indicates a persistent infection in the male reproductive tract is unknown. We hypothesized that if ZIKV establishes a persistent infection in the male reproductive tract (MRT), then immunosuppressant treatment should stimulate ZIKV replication and seminal shedding. Male mice were infected with ZIKV and immunosuppressed when they shed viral RNA but not infectious virus in ejaculates. Following immunosuppression, we did not detect infectious virus in ejaculates. However, we did detect ZIKV positive and negative sense RNA in the epididymal lumens of mice treated with cyclophosphamide, suggesting that ZIKV persists in the epididymis. This study provides insight into the mechanisms behind ZIKV sexual transmission, which may inform public health decisions regarding ZIKV risks.
Identifiants
pubmed: 34023724
pii: S0042-6822(21)00105-7
doi: 10.1016/j.virol.2021.05.001
pmc: PMC8206041
mid: NIHMS1709025
pii:
doi:
Substances chimiques
Immunosuppressive Agents
0
RNA, Viral
0
Cyclophosphamide
8N3DW7272P
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
43-53Subventions
Organisme : NIAID NIH HHS
ID : R21 AI142504
Pays : United States
Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Références
Epidemics. 2016 Dec;17:50-55
pubmed: 27846442
J Infect Dis. 2020 Apr 7;221(9):1506-1517
pubmed: 31616920
Cell. 2016 Dec 1;167(6):1511-1524.e10
pubmed: 27884405
J Gen Virol. 1986 Feb;67 ( Pt 2):381-5
pubmed: 3003242
MMWR Morb Mortal Wkly Rep. 2020 Jan 24;69(3):67-71
pubmed: 31971935
Mol Cell Endocrinol. 2011 Mar 15;335(1):2-13
pubmed: 20398732
Emerg Infect Dis. 2015 Feb;21(2):359-61
pubmed: 25625872
J Virol. 2017 Oct 27;91(22):
pubmed: 28878076
PLoS Negl Trop Dis. 2016 May 05;10(5):e0004658
pubmed: 27149521
J Interferon Cytokine Res. 2006 Nov;26(11):804-19
pubmed: 17115899
Pathog Dis. 2014 Jul;71(2):137-63
pubmed: 24737600
Fertil Res Pract. 2019 Jan 3;5:1
pubmed: 30619616
N Engl J Med. 2016 Jun 2;374(22):2195-8
pubmed: 27074370
J Infect Dis. 2010 Jan 1;201(1):2-4
pubmed: 19961306
Am J Pathol. 2018 Apr;188(4):904-915
pubmed: 29378173
Nature. 2016 Dec 15;540(7633):438-442
pubmed: 27798603
PLoS Negl Trop Dis. 2017 Oct 6;11(10):e0005990
pubmed: 28985234
Cell Host Microbe. 2016 May 11;19(5):720-30
pubmed: 27066744
J Infect Dis. 2012 May 1;205(9):1476-7
pubmed: 22438324
Lancet Infect Dis. 2020 Dec;20(12):1371
pubmed: 33248032
Emerg Infect Dis. 2011 May;17(5):880-2
pubmed: 21529401
J Infect Dis. 2018 Jan 30;217(4):538-547
pubmed: 28968863
J Clin Invest. 2018 Oct 1;128(10):4697-4710
pubmed: 30063220
Euro Surveill. 2016 Jun 9;21(23):
pubmed: 27311680
PLoS Negl Trop Dis. 2018 Aug 22;12(8):e0006733
pubmed: 30133450
Sci Adv. 2017 Feb 22;3(2):e1602899
pubmed: 28261663
PLoS Pathog. 2020 Apr 17;16(4):e1008509
pubmed: 32302362
Emerg Infect Dis. 2008 Aug;14(8):1232-9
pubmed: 18680646
Clin Infect Dis. 2018 Sep 14;67(7):1143-1144
pubmed: 29617955
Am J Trop Med Hyg. 2016 Jun 1;94(6):1362-1369
pubmed: 27022155
MMWR Morb Mortal Wkly Rep. 2017 Jun 16;66(23):615-621
pubmed: 28617773
J Infect Dis. 2019 Jan 1;219(1):31-40
pubmed: 30059980
N Engl J Med. 2018 Apr 12;378(15):1377-1385
pubmed: 29641964
Cell Rep. 2017 Feb 14;18(7):1751-1760
pubmed: 28199846
Biol Reprod. 2011 May;84(5):851-8
pubmed: 21209417
PLoS Negl Trop Dis. 2018 Aug 2;12(8):e0006691
pubmed: 30070988
Emerg Infect Dis. 2019 Aug;25(8):1598-1600
pubmed: 31310210
Immunopharmacology. 2000 May;47(2-3):119-25
pubmed: 10878286
PLoS Med. 2018 Jul 24;15(7):e1002611
pubmed: 30040845
MMWR Morb Mortal Wkly Rep. 2017 Apr 07;66(13):366-373
pubmed: 28384133
Am J Trop Med Hyg. 2017 Nov;97(5):1410-1417
pubmed: 28820694
J Virol. 2000 Jul;74(13):5957-67
pubmed: 10846077
J Immunother Cancer. 2018 Jun 11;6(1):51
pubmed: 29891009
J Virol. 2003 Jan;77(1):25-36
pubmed: 12477807
Virology. 2019 Sep;535:1-10
pubmed: 31254742
Vet Parasitol. 2007 Nov 10;149(3-4):147-57
pubmed: 17904293
JAMA. 2017 Jan 3;317(1):59-68
pubmed: 27960197
Clin Exp Immunol. 1991 Jul;85(1):85-9
pubmed: 1649022
Sci Rep. 2016 Jun 17;6:28070
pubmed: 27312324
J Virol. 2019 Dec 12;94(1):
pubmed: 31597777
Semin Arthritis Rheum. 1992 Jun;21(6 Suppl 3):16-22
pubmed: 1502562
Emerg Infect Dis. 2017 May;23(5):863-865
pubmed: 28257281
PLoS Negl Trop Dis. 2016 Apr 19;10(4):e0004682
pubmed: 27093158
Cell Mol Immunol. 2014 Sep;11(5):428-37
pubmed: 24954222
Sci Rep. 2018 Mar 14;8(1):4510
pubmed: 29540804
Front Immunol. 2012 Jun 11;3:152
pubmed: 22701457
Vet Parasitol. 2006 Oct 10;141(1-2):66-83
pubmed: 16757117
Arch Virol. 1983;75(1-2):71-86
pubmed: 6299247
PLoS Negl Trop Dis. 2017 Jan 9;11(1):e0005296
pubmed: 28068342
Clin Infect Dis. 2018 Apr 3;66(8):1173-1180
pubmed: 29300893
MMWR Morb Mortal Wkly Rep. 2016 Mar 04;65(8):215-6
pubmed: 26937739
Sci Rep. 2018 Apr 3;8(1):5477
pubmed: 29615760
Anticancer Res. 2014 Dec;34(12):7177-83
pubmed: 25503146
PLoS One. 2010 May 14;5(5):e10649
pubmed: 20498839