High-Throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains.
Confocal fluorescence microscopy
Genetic screening
High-content screening
High-throughput microscopy
Yeast
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
24
5
2021
pubmed:
25
5
2021
medline:
12
8
2021
Statut:
ppublish
Résumé
We describe a protocol for live-cell high-throughput (HTP) screening of yeast mutant strains carrying fluorescent protein markers for subcellular compartments of choice using automated confocal microscopy. This procedure, which combines HTP genetics and microscopy, results in the acquisition of thousands of images that can be analyzed in a systematic and quantitative way to identify morphology defects in the tagged subcellular compartments. This HTP protocol is readily adapted for screening any combination of markers and can be expanded to different growth conditions or higher order mutant genetic backgrounds.
Identifiants
pubmed: 34028720
doi: 10.1007/978-1-0716-1402-0_12
pmc: PMC9107603
mid: NIHMS1805108
doi:
Substances chimiques
Fluorescent Dyes
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
221-242Subventions
Organisme : NHGRI NIH HHS
ID : R01 HG005853
Pays : United States
Organisme : CIHR
ID : FDN-143264
Pays : Canada
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