Analysis of unigenes involved in lateral root development in Bupleurum chinense and B. scorzonerifolium.
Auxin signal transduction
Bupleurum
Lateral roots
Transcription factors
Transcriptome analysis
Journal
Planta
ISSN: 1432-2048
Titre abrégé: Planta
Pays: Germany
ID NLM: 1250576
Informations de publication
Date de publication:
26 May 2021
26 May 2021
Historique:
received:
08
04
2021
accepted:
19
05
2021
entrez:
26
5
2021
pubmed:
27
5
2021
medline:
29
5
2021
Statut:
epublish
Résumé
We identified IAA13 negatively associated with lateral root number by comparing the differential expressed genes between Bupleurum chinense and B. scorzonerifolium. Dried roots of the genus Bupleurum L. are used as a herbal medicine for diseases in Asia. Bupleurum chinense has a greater number of lateral roots than B. scorzonerifolium, but the genetic mechanisms for such differences are largely unknown. We (a) compared the transcriptome profiles of the two species and (b) identified a subset of candidate genes involved in auxin signal transduction and explored their functions in lateral root development. By isoform sequencing (Iso-Seq) analyses of the whole plant, more unigenes were found in B. scorzonerifolium (118,868) than in B. chinense (93,485). Given the overarching role of indole-3-acetic acid (IAA) as one of the major regulators of lateral root development, we identified 539 unigenes associated with auxin signal transduction. Fourteen and 44 unigenes in the pathway were differentially expressed in B. chinense and B. scorzonerifolium, respectively, and 3 unigenes (LAX2, LAX4, and IAA13) were expressed in both species. The number of lateral root primordia increased after exogenous auxin application at 8 h and 12 h in B. scorzonerifolium and B. chinense, respectively. Since overexpression of IAA13 in Arabidopsis reduced the number of lateral roots, we hypothesized that IAA13 is involved in the reduction of the number of lateral roots in B. scorzonerifolium.
Identifiants
pubmed: 34037846
doi: 10.1007/s00425-021-03644-x
pii: 10.1007/s00425-021-03644-x
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
128Subventions
Organisme : National Natural Science Foundation of China
ID : 81603223
Organisme : China Agriculture Research System
ID : CARS-21
Organisme : CAMS Innovation Fund for Medical Sciences (CIFMS)
ID : 2016-I2M-2-003
Organisme : the National Transgenic Major Project of China
ID : 2019ZX08010004-005
Organisme : the Crop and livestock Breeding Project in Sichuan
ID : 2021YFYZ0012
Organisme : the Programs of Science and Technology Department of Sichuan Province
ID : 2019YFH0072 and 2019YJ0443
Organisme : the Doctoral research funding of Southwest University of Science and Technology
ID : 19zx7117
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