Risk awareness during operation of analytical flow cytometers and implications throughout the COVID-19 pandemic.
COVID‐19
Core Laboratory
SARS‐CoV‐2
SRL
Shared Resource Laboratory
aerosols
biosafety guidelines
flow cytometry
pandemic
risk assessment
Journal
Cytometry. Part A : the journal of the International Society for Analytical Cytology
ISSN: 1552-4930
Titre abrégé: Cytometry A
Pays: United States
ID NLM: 101235694
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
revised:
24
11
2020
received:
16
09
2020
accepted:
01
12
2020
entrez:
26
5
2021
pubmed:
27
5
2021
medline:
3
6
2021
Statut:
ppublish
Résumé
The COVID-19 pandemic has brought biosafety to the forefront of many life sciences. The outbreak has compelled research institutions to re-evaluate biosafety practices and potential at-risk areas within research laboratories and more specifically within Shared Resource Laboratories (SRLs). In flow cytometry facilities, biological safety assessment encompasses known hazards based on the biological sample and associated risk group, as well as potential or unknown hazards, such as aerosol generation and instrument "failure modes." Cell sorting procedures undergo clearly defined biological safety assessments and adhere to well-established biosafety guidelines that help to protect SRL staff and users against aerosol exposure. Conversely, benchtop analyzers are considered low risk due to their low sample pressure and enclosed fluidic systems, although there is little empirical evidence to support this assumption of low risk. To investigate this, we evaluated several regions on analyzers using the Cyclex-d microsphere assay, a recently established method for cell sorter aerosol containment testing. We found that aerosol and/or droplet hazards were detected on all benchtop analyzers predominantly during operation in "failure modes." These results indicate that benchtop analytical cytometers present a more complicated set of risks than are commonly appreciated.
Identifiants
pubmed: 34038035
doi: 10.1002/cyto.a.24282
pmc: PMC10493867
mid: NIHMS1926317
doi:
Substances chimiques
Aerosols
0
Types de publication
Comparative Study
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
81-89Subventions
Organisme : Intramural NIH HHS
ID : Z99 AI999999
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIC AI001313
Pays : United States
Informations de copyright
© 2020 International Society for Advancement of Cytometry.
Références
Cytometry A. 2014 May;85(5):434-53
pubmed: 24634405
Cytometry. 1997 Jun 1;28(2):97-8
pubmed: 9181298
Clin Infect Dis. 2010 Nov 15;51(10):1176-83
pubmed: 20942655
MMWR Suppl. 1987 Aug 21;36(2):1S-18S
pubmed: 3112554
Cytometry A. 2020 Jul;97(7):674-680
pubmed: 32488957
Cytometry A. 2019 Feb;95(2):173-182
pubmed: 30561906
Can J Med Technol. 1988 Feb;50(1):49-59
pubmed: 10286592
J Hosp Infect. 2006 Oct;64(2):100-14
pubmed: 16916564
MMWR Recomm Rep. 1994 Mar 4;43(RR-3):1-21
pubmed: 7908403
Cytometry A. 2021 Jan;99(1):68-80
pubmed: 33289290
Ann N Y Acad Sci. 1980;353:147-56
pubmed: 6261640
N Engl J Med. 2020 Apr 16;382(16):1564-1567
pubmed: 32182409
Cytometry A. 2016 Nov;89(11):1017-1030
pubmed: 27813253
Cancer Cytopathol. 2020 May;128(5):309-316
pubmed: 32259402
J Hosp Infect. 2020 Oct;106(2):226-231
pubmed: 32652214
Viruses. 2020 Jun 08;12(6):
pubmed: 32521706
Cytometry A. 2011 Dec;79(12):1000-8
pubmed: 22052694
Virology. 2020 Sep;548:39-48
pubmed: 32838945
Gates Open Res. 2018 Feb 27;1:2
pubmed: 29608197
Cytometry A. 2021 Jan;99(1):42-50
pubmed: 33175460
Viruses. 2020 Jun 06;12(6):
pubmed: 32517266
Am J Infect Control. 2007 Dec;35(10 Suppl 2):S65-164
pubmed: 18068815
Lancet Microbe. 2020 May;1(1):e10
pubmed: 32835322
Am J Epidemiol. 1978 May;107(5):421-32
pubmed: 665658
Lancet. 2004 May 15;363(9421):1566-8
pubmed: 15145625
Am J Public Health. 1990 Apr;80(4):423-7
pubmed: 2316762