ELAVL1 primarily couples mRNA stability with the 3' UTRs of interferon-stimulated genes.
3′ UTR
ELAVL1
HuR
RNA stability
RNA-binding protein
binding site
innate immunity
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
25 05 2021
25 05 2021
Historique:
received:
17
09
2020
revised:
13
03
2021
accepted:
04
05
2021
entrez:
26
5
2021
pubmed:
27
5
2021
medline:
11
2
2022
Statut:
ppublish
Résumé
Upon pathogen detection, the innate immune system triggers signaling events leading to upregulation of pro-inflammatory and anti-microbial mRNA transcripts. RNA-binding proteins (RBPs) interact with these critical mRNAs and regulate their fates at the post-transcriptional level. One such RBP is ELAVL1. Although significant progress has been made in understanding how embryonic lethal vision-like protein 1 (ELAVL1) regulates mRNAs, its target repertoire and binding distribution within an immunological context remain poorly understood. We overlap four high-throughput approaches to define its context-dependent targets and determine its regulatory impact during immune activation. ELAVL1 transitions from binding overwhelmingly intronic sites to 3' UTR sites upon immune stimulation of cells, binding previously and newly expressed mRNAs. We find that ELAVL1 mediates the RNA stability of genes that regulate pathways essential to pathogen sensing and cytokine production. Our findings reveal the importance of examining RBP regulatory impact under dynamic transcriptomic events to understand their post-transcriptional regulatory roles within specific biological circuitries.
Identifiants
pubmed: 34038724
pii: S2211-1247(21)00523-4
doi: 10.1016/j.celrep.2021.109178
pmc: PMC8225249
mid: NIHMS1708606
pii:
doi:
Substances chimiques
3' Untranslated Regions
0
ELAV-Like Protein 1
0
RNA, Messenger
0
Interferons
9008-11-1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
109178Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM119569
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM065086
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002243
Pays : United States
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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