Human stem cells harboring a suicide gene improve the safety and standardisation of neural transplants in Parkinsonian rats.
Animals
Apoptosis
/ genetics
Cell Differentiation
Cell Engineering
/ methods
Cell Line
Cell Proliferation
/ genetics
Disease Models, Animal
Dopaminergic Neurons
/ physiology
Female
Genes, Transgenic, Suicide
Genes, bcl-1
/ genetics
Heterografts
/ cytology
Human Embryonic Stem Cells
/ physiology
Humans
Male
Mesencephalon
/ cytology
Oxidopamine
/ administration & dosage
Parkinson Disease, Secondary
/ chemically induced
Rats
Stem Cell Transplantation
/ adverse effects
Thymidine Kinase
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
27 05 2021
27 05 2021
Historique:
received:
04
10
2020
accepted:
14
04
2021
entrez:
28
5
2021
pubmed:
29
5
2021
medline:
10
6
2021
Statut:
epublish
Résumé
Despite advancements in human pluripotent stem cells (hPSCs) differentiation protocols to generate appropriate neuronal progenitors suitable for transplantation in Parkinson's disease, resultant grafts contain low proportions of dopamine neurons. Added to this is the tumorigenic risk associated with the potential presence of incompletely patterned, proliferative cells within grafts. Here, we utilised a hPSC line carrying a FailSafe
Identifiants
pubmed: 34045451
doi: 10.1038/s41467-021-23125-9
pii: 10.1038/s41467-021-23125-9
pmc: PMC8160354
doi:
Substances chimiques
Oxidopamine
8HW4YBZ748
Thymidine Kinase
EC 2.7.1.21
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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