A Mouse Model of Necrotizing Enterocolitis.
Animals
Animals, Newborn
Disease Models, Animal
Enterocolitis, Necrotizing
/ pathology
Humans
Infant, Newborn
Infant, Newborn, Diseases
/ pathology
Inflammation
/ pathology
Intestinal Mucosa
/ pathology
Intestines
/ pathology
Mice
Mice, Inbred C57BL
Premature Birth
/ pathology
Transcription, Genetic
/ physiology
Enteric bacteria
Intestinal inflammation
Mucosal barrier
Necrotizing enterocolitis
Neonatal mice
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
28
5
2021
pubmed:
29
5
2021
medline:
11
8
2021
Statut:
ppublish
Résumé
Necrotizing enterocolitis (NEC) is an acute inflammatory disease that unforeseeably develops in very low birth weight premature infants. NEC is characterized by impairment of the intestinal barrier resulting in intestinal necrosis and multisystem organ failure. Animal models of NEC have contributed significantly to a better understanding of the underlying molecular mechanisms of the disease and facilitated the exploration of potential new therapeutic strategies. Here, we provide a detailed protocol that recapitulates some of the main histological and transcriptional features of human NEC in newborn mice.
Identifiants
pubmed: 34048010
doi: 10.1007/978-1-0716-1488-4_9
pmc: PMC8272439
mid: NIHMS1717030
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101-110Subventions
Organisme : NIDDK NIH HHS
ID : K08 DK101608
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK118568
Pays : United States
Organisme : NIDDK NIH HHS
ID : R03 DK111473
Pays : United States
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