All-in-One DNA Extraction Tube for Facilitated Real-Time Detection of Infectious Pathogens.
all-in-one tubes
nucleic acid extraction
pathogen detection
real-time polymerase chain reaction
tertiary-amine-containing functional polymers
Journal
Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
revised:
29
04
2021
received:
07
03
2021
pubmed:
30
5
2021
medline:
6
8
2021
entrez:
29
5
2021
Statut:
ppublish
Résumé
An "all-in-one tube" platform is developed, where the genetic analysis involving DNA extraction, amplification, and detection can be performed in a single tube. The all-in-one tube consists of a polymerase chain reaction (PCR) tube in which the inner surface is conformally modified with a tertiary-amine-containing polymer to generate a strong electrostatic interaction with DNA. The all-in-one tube provides high DNA capture efficiency exceeding 80% from Escherichia coli O157: H7 pathogen at a wide range of DNA amount from 0.003 to 3 ng. Indeed, the use of the surface-functionalized PCR tube enables direct amplification and detection of the surface-captured DNA without the modification of standard real-time PCR instrument. Besides, this platform has sensitivity, selectivity, and reliability enough for accurate detection at the minimal infective dose of both gram-positive and negative pathogens. The all-in-one tube enables the direct molecular diagnosis, substantially reducing the labor-intensive pathogen detection steps while providing high compatibility with the currently established real-time PCR instruments, and illustrates its on-site applicability with convenience expandable to various genetic analyses including food safety testing, forensic analysis, and clinical diagnosis.
Identifiants
pubmed: 34050626
doi: 10.1002/adhm.202100430
doi:
Substances chimiques
DNA, Bacterial
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2100430Informations de copyright
© 2021 Wiley-VCH GmbH.
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