The phenylpropanoid pathway inhibitor piperonylic acid induces broad-spectrum pest and disease resistance in plants.
Animals
Benzoates
/ pharmacology
Botrytis
Disease Resistance
/ drug effects
Flavonoids
/ metabolism
Gene Expression Profiling
Solanum lycopersicum
/ drug effects
Metabolic Networks and Pathways
/ drug effects
Nematoda
/ metabolism
Plant Diseases
/ immunology
Plant Growth Regulators
/ metabolism
Plant Roots
/ immunology
Pseudomonas syringae
Transcriptome
flavonoids
induced resistance
metabolomics
plant immunity
salicylic acid
tomato
transcriptomics
Journal
Plant, cell & environment
ISSN: 1365-3040
Titre abrégé: Plant Cell Environ
Pays: United States
ID NLM: 9309004
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
10
03
2021
accepted:
23
05
2021
pubmed:
31
5
2021
medline:
15
12
2021
entrez:
30
5
2021
Statut:
ppublish
Résumé
Although many phenylpropanoid pathway-derived molecules act as physical and chemical barriers to pests and pathogens, comparatively little is known about their role in regulating plant immunity. To explore this research field, we transiently perturbed the phenylpropanoid pathway through application of the CINNAMIC ACID-4-HYDROXYLASE (C4H) inhibitor piperonylic acid (PA). Using bioassays involving diverse pests and pathogens, we show that transient C4H inhibition triggers systemic, broad-spectrum resistance in higher plants without affecting growth. PA treatment enhances tomato (Solanum lycopersicum) resistance in field and laboratory conditions, thereby illustrating the potential of phenylpropanoid pathway perturbation in crop protection. At the molecular level, transcriptome and metabolome analyses reveal that transient C4H inhibition in tomato reprograms phenylpropanoid and flavonoid metabolism, systemically induces immune signalling and pathogenesis-related genes, and locally affects reactive oxygen species metabolism. Furthermore, C4H inhibition primes cell wall modification and phenolic compound accumulation in response to root-knot nematode infection. Although PA treatment induces local accumulation of the phytohormone salicylic acid, the PA resistance phenotype is preserved in tomato plants expressing the salicylic acid-degrading NahG construct. Together, our results demonstrate that transient phenylpropanoid pathway perturbation is a conserved inducer of plant resistance and thus highlight the crucial regulatory role of this pathway in plant immunity.
Substances chimiques
Benzoates
0
Flavonoids
0
Plant Growth Regulators
0
piperonylic acid
QX3V1NO0KH
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3122-3139Informations de copyright
© 2021 John Wiley & Sons Ltd.
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