The Vacuole and Mitochondria Patch (vCLAMP) Protein Vam6 is Crucial for Autophagy in Candida albicans.
Autophagy
Candida albicans
Vam6
vCLAMP
Journal
Mycopathologia
ISSN: 1573-0832
Titre abrégé: Mycopathologia
Pays: Netherlands
ID NLM: 7505689
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
23
12
2020
accepted:
24
05
2021
pubmed:
1
6
2021
medline:
1
10
2021
entrez:
31
5
2021
Statut:
ppublish
Résumé
Vacuole and mitochondria patches (vCLAMPs) are involved in the stress resistance of yeast, but their exact role in autophagy remains so far unclear. This study, for the first time, investigated the role of the vCLAMP core protein Vam6 in autophagy of Candida albicans. The experiments demonstrated that the deletion of VAM6 led to a growth defect under nitrogen starvation. Also, western blotting revealed that the vam6Δ/Δ mutant attenuated degradation of Atg8 (an autophagy indicator), Lap41 (an indicator of the cytoplasm to vacuole targeting pathway), and Csp37 (a mitophagy indicator). Moreover, the activity of carboxypeptidase Y and the levels of the vacuolar phospholipase Atg15 were significantly decreased in the mutant, which confirmed the defect of autophagy caused by deletion of VAM6. Overall, these results revealed that Vam6 is essential in maintaining the autophagic process under nitrogen starvation, and this provided new insights into the correlation between vCLAMPs and autophagy.
Identifiants
pubmed: 34057669
doi: 10.1007/s11046-021-00565-x
pii: 10.1007/s11046-021-00565-x
doi:
Substances chimiques
Fungal Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
477-486Subventions
Organisme : National Natural Science Foundation of China
ID : 32070145, 81873961, 31870139
Organisme : Natural Science Foundation of Tianjin City
ID : 17JCZDJC33300
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.
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