Megakaryocyte production is sustained by direct differentiation from erythromyeloid progenitors in the yolk sac until midgestation.
Animals
Cell Differentiation
/ physiology
Cell Lineage
/ physiology
Cells, Cultured
Embryo, Mammalian
/ cytology
Erythrocytes
/ cytology
Female
Granulocytes
/ cytology
Hematopoiesis
/ physiology
Hematopoietic Stem Cells
/ cytology
Macrophages
/ cytology
Male
Megakaryocytes
/ cytology
Mice
Mice, Inbred C57BL
Monocytes
/ cytology
Multipotent Stem Cells
/ cytology
Myeloid Cells
/ cytology
Pregnancy
Stem Cells
/ cytology
Yolk Sac
/ cytology
embryo
erythropoiesis
fate mapping
fetal liver
hematopoiesis
mast cells
megakaryopoiesis
resident macrophages
scRNAseq
yolk sac
Journal
Immunity
ISSN: 1097-4180
Titre abrégé: Immunity
Pays: United States
ID NLM: 9432918
Informations de publication
Date de publication:
13 07 2021
13 07 2021
Historique:
received:
03
11
2020
revised:
23
02
2021
accepted:
28
04
2021
pubmed:
2
6
2021
medline:
15
9
2021
entrez:
1
6
2021
Statut:
ppublish
Résumé
The extra-embryonic yolk sac contains the first definitive multipotent hematopoietic cells, denominated erythromyeloid progenitors. They originate in situ prior to the emergence of hematopoietic stem cells and give rise to erythroid, monocytes, granulocytes, mast cells and macrophages, the latter in a Myb transcription factor-independent manner. We uncovered here the heterogeneity of yolk sac erythromyeloid progenitors, at the single cell level, and discriminated multipotent from committed progenitors, prior to fetal liver colonization. We identified two temporally distinct megakaryocyte differentiation pathways. The first occurs in the yolk sac, bypasses intermediate bipotent megakaryocyte-erythroid progenitors and, similar to the differentiation of macrophages, is Myb-independent. By contrast, the second originates later, from Myb-dependent bipotent progenitors expressing Csf2rb and colonize the fetal liver, where they give rise to megakaryocytes and to large numbers of erythrocytes. Understanding megakaryocyte development is crucial as they play key functions during vascular development, in particular in separating blood and lymphatic networks.
Identifiants
pubmed: 34062116
pii: S1074-7613(21)00188-6
doi: 10.1016/j.immuni.2021.04.026
pmc: PMC8284597
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1433-1446.e5Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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