Optimizing Chitin Depolymerization by Lysozyme to Long-Chain Oligosaccharides.
chemo-enzymatic synthesis
chitin oligosaccharides
lysozyme
response surface methodology
Journal
Marine drugs
ISSN: 1660-3397
Titre abrégé: Mar Drugs
Pays: Switzerland
ID NLM: 101213729
Informations de publication
Date de publication:
31 May 2021
31 May 2021
Historique:
received:
18
05
2021
revised:
27
05
2021
accepted:
28
05
2021
entrez:
2
6
2021
pubmed:
3
6
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Chitin oligosaccharides (COs) hold high promise as organic fertilizers in the ongoing agro-ecological transition. Short- and long-chain COs can contribute to the establishment of symbiotic associations between plants and microorganisms, facilitating the uptake of soil nutrients by host plants. Long-chain COs trigger plant innate immunity. A fine investigation of these different signaling pathways requires improving the access to high-purity COs. Here, we used the response surface methodology to optimize the production of COs by enzymatic hydrolysis of water-soluble chitin (WSC) with hen egg-white lysozyme. The influence of WSC concentration, its acetylation degree, and the reaction time course were modelled using a Box-Behnken design. Under optimized conditions, water-soluble COs up to the nonasaccharide were formed in 51% yield and purified to homogeneity. This straightforward approach opens new avenues to determine the complex roles of COs in plants.
Identifiants
pubmed: 34072871
pii: md19060320
doi: 10.3390/md19060320
pmc: PMC8229320
pii:
doi:
Substances chimiques
Oligosaccharides
0
Chitin
1398-61-4
hen egg lysozyme
EC 3.2.1.-
Muramidase
EC 3.2.1.17
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR 14-CE18-0008
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