Pyramiding the antimicrobial PR1aCB and AATCB genes in 'Tarocco' blood orange (Citrus sinensis Osbeck) to enhance citrus canker resistance.
AATCB
Citrus
Citrus canker
Co-transformation
PR1aCB
Re-transformation
Journal
Transgenic research
ISSN: 1573-9368
Titre abrégé: Transgenic Res
Pays: Netherlands
ID NLM: 9209120
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
01
09
2020
accepted:
17
03
2021
pubmed:
3
6
2021
medline:
14
1
2022
entrez:
2
6
2021
Statut:
ppublish
Résumé
Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is a major bacterial disease responsible for substantial economic losses in citrus-producing areas. To breed transgenic citrus plants with enhanced resistance to citrus canker, two antimicrobial peptide genes, PR1aCB and AATCB, were incorporated into 'Tarocco' blood orange (Citrus sinensis Osbeck) plants via co-transformation and sequential re-transformation. The presence of PR1aCB and AATCB in double transgenic plants was confirmed by PCR. The expression of PR1aCB and AATCB in double transformants was demonstrated by quantitative real-time PCR. An in vivo disease resistance assay involving the injection of Xcc revealed that the double transformants were more resistant to citrus canker than the single gene transformants and wild-type plants. An analysis of the bacterial population indicated that the enhanced citrus canker resistance of the double transformants was due to inhibited Xcc growth. These results proved that the pyramiding of multiple genes is a more effective strategy for increasing the disease resistance of transgenic citrus plants than single gene transformations.
Identifiants
pubmed: 34076822
doi: 10.1007/s11248-021-00245-y
pii: 10.1007/s11248-021-00245-y
doi:
Substances chimiques
Anti-Infective Agents
0
Antimicrobial Peptides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
635-647Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
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