Mfge8 attenuates human gastric antrum smooth muscle contractions.
Actins
Mfge8
Myosin light chain phosphatase
Smooth muscle
Stomach
α8 integrin
Journal
Journal of muscle research and cell motility
ISSN: 1573-2657
Titre abrégé: J Muscle Res Cell Motil
Pays: Netherlands
ID NLM: 8006298
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
28
03
2021
accepted:
21
05
2021
pubmed:
5
6
2021
medline:
18
1
2022
entrez:
4
6
2021
Statut:
ppublish
Résumé
Coordinated gastric smooth muscle contraction is critical for proper digestion and is adversely affected by a number of gastric motility disorders. In this study we report that the secreted protein Mfge8 (milk fat globule-EGF factor 8) inhibits the contractile responses of human gastric antrum muscles to cholinergic stimuli by reducing the inhibitory phosphorylation of the MYPT1 (myosin phosphatase-targeting subunit (1) subunit of MLCP (myosin light chain phosphatase), resulting in reduced LC20 (smooth muscle myosin regulatory light chain (2) phosphorylation. Mfge8 reduced the agonist-induced increase in the F-actin/G-actin ratios of β-actin and γ-actin1. We show that endogenous Mfge8 is bound to its receptor, α8β1 integrin, in human gastric antrum muscles, suggesting that human gastric antrum muscle mechanical responses are regulated by Mfge8. The regulation of gastric antrum smooth muscles by Mfge8 and α8 integrin functions as a brake on gastric antrum mechanical activities. Further studies of the role of Mfge8 and α8 integrin in regulating gastric antrum function will likely reveal additional novel aspects of gastric smooth muscle motility mechanisms.
Identifiants
pubmed: 34085177
doi: 10.1007/s10974-021-09604-y
pii: 10.1007/s10974-021-09604-y
pmc: PMC8332633
doi:
Substances chimiques
Antigens, Surface
0
MFGE8 protein, human
0
Milk Proteins
0
Myosin Light Chains
0
Myosin-Light-Chain Phosphatase
EC 3.1.3.53
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
219-231Subventions
Organisme : NIDDK NIH HHS
ID : U24 DK076169
Pays : United States
Informations de copyright
© 2021. The Author(s).
Références
FASEB J. 2018 May 15;:fj201800109R
pubmed: 29763381
J Physiol. 2003 Feb 1;546(Pt 3):879-89
pubmed: 12563012
J Muscle Res Cell Motil. 2013 May;34(2):137-49
pubmed: 23576331
J Biol Chem. 2013 Mar 15;288(11):7596-7605
pubmed: 23362260
J Physiol. 2018 Jun;596(11):2131-2146
pubmed: 29528115
Am J Physiol Cell Physiol. 2008 Sep;295(3):C768-78
pubmed: 18596213
J Physiol. 2015 Feb 1;593(3):681-700
pubmed: 25433069
FEBS Lett. 2002 Sep 11;527(1-3):101-4
pubmed: 12220642
FASEB J. 1989 Sep;3(11):2266-76
pubmed: 2506092
Biochem Biophys Res Commun. 2008 Apr 25;369(1):149-56
pubmed: 18155661
J Biol Chem. 1991 Jan 25;266(3):1708-15
pubmed: 1671041
J Muscle Res Cell Motil. 2012 Dec;33(6):461-9
pubmed: 22311558
Gastroenterology. 2013 Jun;144(7):1456-65, 1465.e1-5
pubmed: 23499953
Arch Biochem Biophys. 2011 Jun 15;510(2):147-59
pubmed: 21291858
Physiol Rev. 2003 Oct;83(4):1325-58
pubmed: 14506307
Am J Physiol Cell Physiol. 2008 Sep;295(3):C576-87
pubmed: 18596210
J Physiol. 1999 Sep 15;519 Pt 3:829-40
pubmed: 10457094
J Biol Chem. 2015 Apr 3;290(14):8677-92
pubmed: 25713079
J Physiol. 2011 Dec 15;589(Pt 24):6105-18
pubmed: 22005683
J Appl Physiol (1985). 2001 Aug;91(2):963-72
pubmed: 11457815
Proc Natl Acad Sci U S A. 2013 Jan 8;110(2):660-5
pubmed: 23269839
Proc Natl Acad Sci U S A. 1990 Nov;87(21):8417-21
pubmed: 2122462
Mol Cell Biochem. 2004 Apr;259(1-2):197-209
pubmed: 15124925
Nat Rev Gastroenterol Hepatol. 2012 Nov;9(11):633-45
pubmed: 22965426
J Physiol. 2018 Aug;596(16):3617-3635
pubmed: 29746010
Neurogastroenterol Motil. 2011 Oct;23(10):e425-36
pubmed: 21883701
J Diabetes Res. 2018 May 31;2018:8743874
pubmed: 29955616
J Med Chem. 2020 Jun 11;63(11):5675-5696
pubmed: 31999923
Neurogastroenterol Motil. 2019 Apr;31(4):e13546
pubmed: 30740834
Curr Opin Gastroenterol. 2010 Nov;26(6):647-55
pubmed: 20838344
Cell. 1997 Mar 7;88(5):603-13
pubmed: 9054500
J Biol Chem. 1999 Dec 24;274(52):37385-90
pubmed: 10601309
J Cell Biochem. 2009 Apr 15;106(6):957-66
pubmed: 19204935
Am J Physiol Cell Physiol. 2008 Aug;295(2):C358-64
pubmed: 18524939
J Biol Chem. 2001 Oct 26;276(43):39858-63
pubmed: 11517233
Cardiovasc Res. 2006 Jul 1;71(1):170-8
pubmed: 16603140
J Biol Chem. 1998 Jan 23;273(4):2384-9
pubmed: 9442086
Novartis Found Symp. 2002;246:228-38; discussion 238-43, 272-6
pubmed: 12164311
Histochem Cell Biol. 2016 Feb;145(2):119-30
pubmed: 26708152
Gastroenterology. 2008 Aug;135(2):610-20
pubmed: 18586037
J Biochem. 1995 Dec;118(6):1104-7
pubmed: 8720121
J Cell Biochem. 2015 Nov;116(11):2667-75
pubmed: 25923522
J Physiol. 2013 Jun 15;591(12):2971-86
pubmed: 23613531
J Cardiovasc Pharmacol. 1986;8 Suppl 8:S42-7
pubmed: 2433524
Am J Hum Genet. 2014 Feb 6;94(2):288-94
pubmed: 24439109
J Biol Chem. 1995 Sep 29;270(39):23196-202
pubmed: 7559467
Aliment Pharmacol Ther. 2011 Apr;33(8):880-94
pubmed: 21342212
Anal Biochem. 2019 Jul 15;577:1-13
pubmed: 30981700
Mol Biol Cell. 2005 Dec;16(12):5528-37
pubmed: 16195353
Expert Opin Ther Targets. 2013 Nov;17(11):1275-85
pubmed: 23972256
Neurogastroenterol Motil. 2011 Sep;23(9):e356-71
pubmed: 21781228
J Food Sci. 2008 Jun;73(5):R67-80
pubmed: 18577009
Elife. 2016 Apr 19;5:
pubmed: 27092791
Expert Opin Investig Drugs. 2019 Oct;28(10):871-889
pubmed: 31566013
J Neurogastroenterol Motil. 2016 Apr 30;22(2):213-25
pubmed: 26701920
Mol Cell Biol. 1988 Dec;8(12):5224-31
pubmed: 3244353
Eur J Biochem. 1992 Dec 15;210(3):1023-35
pubmed: 1336455
Atherosclerosis. 2007 Dec;195(2):303-12
pubmed: 17275006