Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions.
cryo-electron microscopy
electron transfer
membrane proteins
photosystems
plant biology
Journal
The Biochemical journal
ISSN: 1470-8728
Titre abrégé: Biochem J
Pays: England
ID NLM: 2984726R
Informations de publication
Date de publication:
25 06 2021
25 06 2021
Historique:
received:
19
04
2021
revised:
28
05
2021
accepted:
03
06
2021
pubmed:
5
6
2021
medline:
23
11
2021
entrez:
4
6
2021
Statut:
ppublish
Résumé
Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and electron transfer between PSI and Pc. Structural data at 2.74 Å resolution reveals strong hydrophobic interactions in the plant PSI-Pc ternary complex, leading to exclusion of water molecules from PsaA-PsaB/Pc interface once the PSI-Pc complex forms. Upon oxidation of Pc, a slight tilt of bound oxidized Pc allows water molecules to accommodate the space between Pc and PSI to drive Pc dissociation. Such a scenario is consistent with the six times larger dissociation constant of oxidized as compared with reduced Pc and mechanistically explains how this molecular machine optimized electron transfer for fast turnover.
Identifiants
pubmed: 34085703
pii: 228876
doi: 10.1042/BCJ20210267
pmc: PMC8238519
doi:
Substances chimiques
Photosystem I Protein Complex
0
Plastocyanin
9014-09-9
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2371-2384Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103311
Pays : United States
Informations de copyright
© 2021 The Author(s).
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