A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.

Cryoelectron Microscopy RNA, Ribosomal, 16S / genetics Ribosome Subunits, Small Ribosome Subunits, Small, Bacterial Ribosomes

Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
06 2021
Historique:
received: 17 11 2020
accepted: 20 04 2021
entrez: 5 6 2021
pubmed: 6 6 2021
medline: 9 4 2022
Statut: epublish

Résumé

While a structural description of the molecular mechanisms guiding ribosome assembly in eukaryotic systems is emerging, bacteria use an unrelated core set of assembly factors for which high-resolution structural information is still missing. To address this, we used single-particle cryo-electron microscopy to visualize the effects of bacterial ribosome assembly factors RimP, RbfA, RsmA, and RsgA on the conformational landscape of the 30

Identifiants

DOI: 10.1126/sciadv.abf7547 PMID: 34088665 PMC: PMC8177701
pubmed: 34088665
pii: 7/23/eabf7547
doi: 10.1126/sciadv.abf7547
pmc: PMC8177701
pii:
doi:

Substances chimiques

RNA, Ribosomal, 16S 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom

Informations de copyright

Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

Références

Prog Nucl Magn Reson Spectrosc. 2013 Aug;73:1-16
pubmed: 23962882
J Chem Theory Comput. 2008 Jan;4(1):116-22
pubmed: 26619985
Biochem J. 2017 Jun 13;474(13):2145-2158
pubmed: 28512204
Biochemistry. 2011 Apr 19;50(15):3272-8
pubmed: 21410291
J Neurochem. 2019 Feb;148(3):325-347
pubmed: 30144322
Anal Chem. 2001 Aug 1;73(15):3528-34
pubmed: 11510814
Nature. 2000 Sep 21;407(6802):340-8
pubmed: 11014183
Nucleic Acids Res. 2013 Feb 1;41(4):2609-20
pubmed: 23293003
J Biomol NMR. 2016 Oct;66(2):111-124
pubmed: 27663422
Structure. 2007 Mar;15(3):289-97
pubmed: 17355865
Nat Methods. 2017 Aug;14(8):793-796
pubmed: 28671674
Chem Rev. 2009 Mar 11;109(3):839-60
pubmed: 19196002
Nucleic Acids Res. 2001 Feb 1;29(3):638-43
pubmed: 11160884
EMBO J. 2011 Jan 5;30(1):104-14
pubmed: 21102555
Bioinformatics. 2015 Apr 15;31(8):1325-7
pubmed: 25505092
Science. 2010 Oct 29;330(6004):673-7
pubmed: 21030658
J Mol Biol. 1986 Oct 5;191(3):483-93
pubmed: 2434656
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Cell Mol Life Sci. 2000 May;57(5):796-808
pubmed: 10892344
FEBS Lett. 1969 Apr;3(1):85-88
pubmed: 11946975
J Struct Biol. 2016 Jan;193(1):1-12
pubmed: 26592709
J Biomol NMR. 2009 Aug;44(4):213-23
pubmed: 19548092
Mol Microbiol. 1996 Sep;21(6):1207-18
pubmed: 8898389
Nat Chem Biol. 2015 Dec;11(12):924-32
pubmed: 26575239
Biochem Soc Trans. 2016 Aug 15;44(4):1035-44
pubmed: 27528749
Nucleic Acids Res. 2017 Feb 17;45(3):1027-1040
pubmed: 28180306
Microbiol Mol Biol Rev. 2009 Mar;73(1):22-35
pubmed: 19258531
J Biol Chem. 2012 Mar 23;287(13):10453-10459
pubmed: 22308031
J Biol Chem. 2019 Jan 4;294(1):372-378
pubmed: 30409901
Mol Cell. 2007 Nov 9;28(3):434-45
pubmed: 17996707
Nucleic Acids Res. 2017 Jun 20;45(11):6945-6959
pubmed: 28482099
Genes Dev. 1995 Mar 1;9(5):626-37
pubmed: 7535280
RNA. 2020 Dec;26(12):2017-2030
pubmed: 32989043
J Biomol NMR. 1995 Nov;6(3):277-93
pubmed: 8520220
J Mol Biol. 2003 Mar 21;327(2):521-36
pubmed: 12628255
Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3396-E3403
pubmed: 28396444
RNA. 2013 Jun;19(6):789-802
pubmed: 23611982
Nature. 2005 Dec 1;438(7068):628-32
pubmed: 16319883
Elife. 2018 Jun 01;7:
pubmed: 29856314
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Protein Sci. 2018 Jan;27(1):26-40
pubmed: 28766807
Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10037-41
pubmed: 11517324
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W407-10
pubmed: 17517781
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
J Mol Biol. 2004 May 28;339(2):337-53
pubmed: 15136037
Nucleic Acids Res. 2020 Jan 10;48(1):359-372
pubmed: 31728529
Elife. 2018 Nov 09;7:
pubmed: 30412051
Elife. 2014 Oct 14;3:
pubmed: 25313868
J Mol Biol. 2008 Feb 8;376(1):92-108
pubmed: 18155048
J Mol Biol. 2010 Apr 23;398(1):1-7
pubmed: 20188109
Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13325-30
pubmed: 25187561
Nature. 2018 Jun;558(7709):249-253
pubmed: 29875412
Commun Biol. 2019 Jun 19;2:218
pubmed: 31240256
J Mol Biol. 2001 Aug 17;311(3):593-604
pubmed: 11493012
3 Biotech. 2019 Apr;9(4):127
pubmed: 30863706
Proc Natl Acad Sci U S A. 2015 Feb 24;112(8):2425-30
pubmed: 25675474
Nat Methods. 2017 Apr;14(4):331-332
pubmed: 28250466
J Biomol NMR. 1998 Feb;11(2):221-6
pubmed: 9679296
Trends Biochem Sci. 1999 Nov;24(11):437-40
pubmed: 10542411
EMBO J. 2018 Apr 3;37(7):
pubmed: 29459436
Nature. 2008 Oct 30;455(7217):1268-72
pubmed: 18784650
Protein Sci. 2018 Jan;27(1):14-25
pubmed: 28710774
Biomol NMR Assign. 2020 Oct;14(2):189-193
pubmed: 32303998

Auteurs

Andreas Schedlbauer (A)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain.
ORCID: 0000-0001-6270-8686

Idoia Iturrioz (I)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain.

Borja Ochoa-Lizarralde (B)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain.

Tammo Diercks (T)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain.
ORCID: 0000-0002-5200-0905

Jorge Pedro López-Alonso (JP)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain.
ORCID: 0000-0003-2081-2607

José Luis Lavin (JL)

Neiker-Tecnalia, Berreaga Kalea 1, 48160 Derio, Spain.
ORCID: 0000-0003-0914-3211

Tatsuya Kaminishi (T)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain.
Department of Genetics, Graduate School of Medicine, Osaka University, Japan.
ORCID: 0000-0001-5056-1074

Retina Çapuni (R)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain.
ORCID: 0000-0002-0823-7470

Neha Dhimole (N)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain.

Elisa de Astigarraga (E)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain.

David Gil-Carton (D)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain.
ORCID: 0000-0003-4241-1302

Paola Fucini (P)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain. sean.connell@gmail.com pfucini@gmail.com.
IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain.
ORCID: 0000-0003-1724-8507

Sean R Connell (SR)

Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 801A, 48160 Derio, Spain. sean.connell@gmail.com pfucini@gmail.com.
IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain.
ORCID: 0000-0001-7807-7049

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › A conserved rRNA switch is central to decoding...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › A conserved rRNA switch is central to decoding...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › Sous-unités du ribosome › Petite sous-unité du ribosome › A conserved rRNA switch is central to decoding...
questionsmedicales.fr › Structures bactériennes › Petite sous-unité du ribosome des bactéries › A conserved rRNA switch is central to decoding...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › A conserved rRNA switch is central to decoding...