Statistical modeling of in vitro pepsin specificity.

Amino Acid Sequence Amino Acids Chromatography, Liquid Endopeptidases / metabolism Models, Statistical Pepsin A / metabolism Peptides / metabolism Proteins / chemistry Proteolysis Substrate Specificity Tandem Mass Spectrometry
Charge Cleavage specificity Hydrophobicity Pepsinolysis Prediction model Structure

Journal

Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639

Informations de publication

Date de publication:
15 Nov 2021
Historique:
received: 04 02 2021
revised: 13 04 2021
accepted: 11 05 2021
pubmed: 6 6 2021
medline: 17 7 2021
entrez: 5 6 2021
Statut: ppublish

Résumé

The specificity of pepsin, the major protease of gastric digestion, has been previously investigated, but only regarding the primary sequence of the protein substrates. The present study aimed to consider in addition physicochemical and structural characteristics, at the molecular and sub-molecular scales. For six different proteins submitted to in vitro gastric digestion, the peptide bonds cleaved were determined from the peptides released and identified by LC-MS/MS. An original statistical approach, based on propensity scores calculated for each amino acid residue on both sides of the peptide bonds, concluded that preferential cleavage occurred after Leu and Phe, and before Ile. Moreover, reliable statistical models developed for predicting peptide bond cleavage, highlighted the predominant role of the amino acid residues at the N-terminal side of the peptide bonds, up to the seventh position (P7 and P7'). The significant influence of hydrophobicity, charge and structural constraints around the peptide bonds was also evidenced.

Identifiants

DOI: 10.1016/j.foodchem.2021.130098 PMID: 34090041
pubmed: 34090041
pii: S0308-8146(21)01104-3
doi: 10.1016/j.foodchem.2021.130098
pii:
doi:

Substances chimiques

Amino Acids 0
Peptides 0
Proteins 0
Endopeptidases EC 3.4.-
Pepsin A EC 3.4.23.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

130098

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Ousmane Suwareh (O)

STLO, INRAE, Institut Agro, 65 rue de Saint-Brieuc, 35042 Rennes, France. Electronic address: ousmane.suwareh@inrae.fr.

David Causeur (D)

IRMAR UMR6625, CNRS, Institut Agro, 65 rue de Saint-Brieuc, 35042 Rennes, France. Electronic address: david.causeur@agrocampus-ouest.fr.

Julien Jardin (J)

STLO, INRAE, Institut Agro, 65 rue de Saint-Brieuc, 35042 Rennes, France. Electronic address: julien.jardin@inrae.fr.

Valérie Briard-Bion (V)

STLO, INRAE, Institut Agro, 65 rue de Saint-Brieuc, 35042 Rennes, France. Electronic address: valerie.briard-bion@inrae.fr.

Steven Le Feunteun (S)

STLO, INRAE, Institut Agro, 65 rue de Saint-Brieuc, 35042 Rennes, France. Electronic address: steven.le-feunteun@inrae.fr.

Stéphane Pezennec (S)

STLO, INRAE, Institut Agro, 65 rue de Saint-Brieuc, 35042 Rennes, France. Electronic address: stephane.pezennec@inrae.fr.

Françoise Nau (F)

STLO, INRAE, Institut Agro, 65 rue de Saint-Brieuc, 35042 Rennes, France. Electronic address: francoise.nau@agrocampus-ouest.fr.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Statistical modeling of in vitro pepsin specificity.
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Statistical modeling of in vitro pepsin specificity.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Statistical modeling of in vitro pepsin specificity.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Endopeptidases › Statistical modeling of in vitro pepsin specificity.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Modèles statistiques › Statistical modeling of in vitro pepsin specificity.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Endopeptidases › Aspartic acid endopeptidases › Pepsine A › Statistical modeling of in vitro pepsin specificity.
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Statistical modeling of in vitro pepsin specificity.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Statistical modeling of in vitro pepsin specificity.
questionsmedicales.fr › Métabolisme › Protéolyse › Statistical modeling of in vitro pepsin specificity.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Statistical modeling of in vitro pepsin specificity.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Statistical modeling of in vitro pepsin specificity.