Heterogeneity in Human Induced Pluripotent Stem Cell-derived Alveolar Epithelial Type II Cells Revealed with ABCA3/SFTPC Reporters.
ATP-Binding Cassette Transporters
/ metabolism
Alveolar Epithelial Cells
/ cytology
Cell Differentiation
/ physiology
Epithelial Cells
/ metabolism
Humans
Induced Pluripotent Stem Cells
/ cytology
Lung
/ metabolism
Pulmonary Alveoli
/ cytology
Pulmonary Surfactant-Associated Protein C
/ metabolism
Pulmonary Surfactant-Associated Proteins
/ metabolism
ATP binding cassette A3
alveolar epithelial cells
gene editing
human pluripotent stem cells
surfactant
Journal
American journal of respiratory cell and molecular biology
ISSN: 1535-4989
Titre abrégé: Am J Respir Cell Mol Biol
Pays: United States
ID NLM: 8917225
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
pubmed:
9
6
2021
medline:
12
10
2021
entrez:
8
6
2021
Statut:
ppublish
Résumé
Alveolar epithelial type 2 cells (AEC2s), the facultative progenitors of lung alveoli, are typically identified through the use of the canonical markers, SFTPC and ABCA3. Self-renewing AEC2-like cells have been generated from human induced pluripotent stem cells (iPSCs) through the use of knock-in SFTPC fluorochrome reporters. However, developmentally, SFTPC expression onset begins in the fetal distal lung bud tip and thus is not specific to mature AEC2s. Furthermore, SFTPC reporters appear to identify only those iPSC-derived AEC2s (iAEC2s) expressing the highest SFTPC levels. Here, we generate an ABCA3 knock-in GFP fusion reporter (ABCA3:GFP) that enables the purification of iAEC2s while allowing visualization of lamellar bodies, organelles associated with AEC2 maturation. Using an SFTPC
Identifiants
pubmed: 34101541
doi: 10.1165/rcmb.2020-0259OC
pmc: PMC8525201
doi:
Substances chimiques
ABCA3 protein, human
0
ATP-Binding Cassette Transporters
0
Pulmonary Surfactant-Associated Protein C
0
Pulmonary Surfactant-Associated Proteins
0
SFTPC protein, human
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
442-460Subventions
Organisme : NCATS NIH HHS
ID : U01 TR001810
Pays : United States
Organisme : NCATS NIH HHS
ID : TL1 TR001410
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL134745
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL145408
Pays : United States
Organisme : NHLBI NIH HHS
ID : F30 HL142169
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL095993
Pays : United States
Organisme : BLRD VA
ID : I01 BX001176
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL148692
Pays : United States
Organisme : NHLBI NIH HHS
ID : 75N92020C00005
Pays : United States
Commentaires et corrections
Type : CommentIn
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