Transglutaminase 2 promotes tumorigenicity of colon cancer cells by inactivation of the tumor suppressor p53.


Journal

Oncogene
ISSN: 1476-5594
Titre abrégé: Oncogene
Pays: England
ID NLM: 8711562

Informations de publication

Date de publication:
06 2021
Historique:
received: 04 11 2020
accepted: 17 05 2021
revised: 30 04 2021
pubmed: 10 6 2021
medline: 22 12 2021
entrez: 9 6 2021
Statut: ppublish

Résumé

Despite a high clinical need for the treatment of colorectal carcinoma (CRC) as the second leading cause of cancer-related deaths, targeted therapies are still limited. The multifunctional enzyme Transglutaminase 2 (TGM2), which harbors transamidation and GTPase activity, has been implicated in the development and progression of different types of human cancers. However, the mechanism and role of TGM2 in colorectal cancer are poorly understood. Here, we present TGM2 as a promising drug target.In primary patient material of CRC patients, we detected an increased expression and enzymatic activity of TGM2 in colon cancer tissue in comparison to matched normal colon mucosa cells. The genetic ablation of TGM2 in CRC cell lines using shRNAs or CRISPR/Cas9 inhibited cell expansion and tumorsphere formation. In vivo, tumor initiation and growth were reduced upon genetic knockdown of TGM2 in xenotransplantations. TGM2 ablation led to the induction of Caspase-3-driven apoptosis in CRC cells. Functional rescue experiments with TGM2 variants revealed that the transamidation activity is critical for the pro-survival function of TGM2. Transcriptomic and protein-protein interaction analyses applying various methods including super-resolution and time-lapse microscopy showed that TGM2 directly binds to the tumor suppressor p53, leading to its inactivation and escape of apoptosis induction.We demonstrate here that TGM2 is an essential survival factor in CRC, highlighting the therapeutic potential of TGM2 inhibitors in CRC patients with high TGM2 expression. The inactivation of p53 by TGM2 binding indicates a general anti-apoptotic function, which may be relevant in cancers beyond CRC.

Identifiants

pubmed: 34103685
doi: 10.1038/s41388-021-01847-w
pii: 10.1038/s41388-021-01847-w
pmc: PMC8225513
doi:

Substances chimiques

TGM2 protein, human 0
TP53 protein, human 0
Tumor Suppressor Protein p53 0
Protein Glutamine gamma Glutamyltransferase 2 EC 2.3.2.13
Caspase 3 EC 3.4.22.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4352-4367

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Auteurs

Patrizia Malkomes (P)

Goethe University Hospital Frankfurt, Department of General, Visceral and Transplant Surgery, Frankfurt am Main, Germany.

Ilaria Lunger (I)

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany.

Elsie Oppermann (E)

Goethe University Hospital Frankfurt, Department of General, Visceral and Transplant Surgery, Frankfurt am Main, Germany.

Khalil Abou-El-Ardat (K)

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany.
German Cancer Consortium and German Cancer Research Center (DKFZ), Heidelberg, Germany.

Thomas Oellerich (T)

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany.
German Cancer Consortium and German Cancer Research Center (DKFZ), Heidelberg, Germany.
Frankfurt Cancer Institute, Frankfurt am Main, Germany.

Stefan Günther (S)

Max Planck Institute for Heart and Lung Research, Department I Cardiac Development and Remodelling, Bad Nauheim, Germany.

Can Canbulat (C)

Goethe University Hospital Frankfurt, Department of General, Visceral and Transplant Surgery, Frankfurt am Main, Germany.

Sabrina Bothur (S)

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany.

Frank Schnütgen (F)

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany.
German Cancer Consortium and German Cancer Research Center (DKFZ), Heidelberg, Germany.
Frankfurt Cancer Institute, Frankfurt am Main, Germany.

Weijia Yu (W)

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany.

Susanne Wingert (S)

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany.

Nadine Haetscher (N)

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany.

Claudia Catapano (C)

Single Molecule Biophysics, Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.

Marina S Dietz (MS)

Single Molecule Biophysics, Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.

Mike Heilemann (M)

Single Molecule Biophysics, Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.

Hans-Michael Kvasnicka (HM)

Goethe University Frankfurt, Senckenberg Institute for Pathology, Frankfurt am Main, Germany.

Katharina Holzer (K)

Goethe University Hospital Frankfurt, Department of General, Visceral and Transplant Surgery, Frankfurt am Main, Germany.
Philipps University of Marburg, Department of Visceral-, Thoracic- and Vascular Surgery, Marburg, Germany.

Hubert Serve (H)

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany.
German Cancer Consortium and German Cancer Research Center (DKFZ), Heidelberg, Germany.
Frankfurt Cancer Institute, Frankfurt am Main, Germany.

Wolf Otto Bechstein (WO)

Goethe University Hospital Frankfurt, Department of General, Visceral and Transplant Surgery, Frankfurt am Main, Germany.

Michael A Rieger (MA)

Goethe University Hospital Frankfurt, Department of Medicine, Hematology/Oncology, Frankfurt am Main, Germany. m.rieger@em.uni-frankfurt.de.
German Cancer Consortium and German Cancer Research Center (DKFZ), Heidelberg, Germany. m.rieger@em.uni-frankfurt.de.
Frankfurt Cancer Institute, Frankfurt am Main, Germany. m.rieger@em.uni-frankfurt.de.
Cardio-Pulmonary Institute, Frankfurt am Main, Germany. m.rieger@em.uni-frankfurt.de.

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